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dc.contributor.authorFang, Xianyang
Michnicka, Malgorzata
Zhang, Yikan
Wang, Yun-Xing
Nikonowicz, Edward P.
dc.date.accessioned 2017-11-14T18:08:24Z
dc.date.available 2017-11-14T18:08:24Z
dc.date.issued 2017
dc.identifier.citation Fang, Xianyang, Michnicka, Malgorzata, Zhang, Yikan, et al.. "Capture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitch." Biochemistry, 56, no. 28 (2017) American Chemical Society: 3549-3558. https://doi.org/10.1021/acs.biochem.7b00284.
dc.identifier.urihttps://hdl.handle.net/1911/98807
dc.description.abstract In Gram-positive bacteria, the tRNA-dependent T-box riboswitch system regulates expression of amino acid biosynthetic and aminoacyl-tRNA synthetase genes through a transcription attenuation mechanism. Binding of uncharged tRNA “closes” the switch, allowing transcription read-through. Structural studies of the 100-nucleotide stem I domain reveal tRNA utilizes base pairing and stacking interactions to bind the stem, but little is known structurally about the 180-nucleotide riboswitch core (stem I, stem III, and antiterminator stem) in complex with tRNA or the mechanism of coupling of the intermolecular binding domains crucial to T-box function. Here we utilize solution structural and biophysical methods to characterize the interplay of the different riboswitch–tRNA contact points using Bacillus subtilis and Oceanobacillus iheyensis glycyl T-box and T-box:tRNA constructs. The data reveal that tRNA:riboswitch core binding at equilibrium involves only Specifier–anticodon and antiterminator–acceptor stem pairing. The elbow:platform stacking interaction observed in studies of the T-box stem I domain is released after pairing between the acceptor stem and the bulge in the antiterminator helix. The results are consistent with the model of T-box riboswitch:tRNA function in which tRNA is captured by stem I of the nascent mRNA followed by stabilization of the antiterminator helix and the paused transcription complex.
dc.language.iso eng
dc.publisher American Chemical Society
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.
dc.title Capture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitch
dc.type Journal article
dc.citation.journalTitle Biochemistry
dc.citation.volumeNumber 56
dc.citation.issueNumber 28
dc.identifier.digital Tbox_SAXS_BCHM_wFIGS
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1021/acs.biochem.7b00284
dc.type.publication post-print
dc.citation.firstpage 3549
dc.citation.lastpage 3558


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