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dc.contributor.authorHe, Ya
Mathieu, Jacques
da Silva, Marcio L.B.
Li, Mengyan
Alvarez, Pedro J.J.
dc.date.accessioned 2017-10-27T13:26:30Z
dc.date.available 2017-10-27T13:26:30Z
dc.date.issued 2017
dc.identifier.citation He, Ya, Mathieu, Jacques, da Silva, Marcio L.B., et al.. "1,4-Dioxane-degrading consortia can be enriched from uncontaminated soils: prevalence of Mycobacterium and soluble di-iron monooxygenase genes." Microbial Biotechnology, (2017) Wiley: https://doi.org/10.1111/1751-7915.12850.
dc.identifier.urihttps://hdl.handle.net/1911/97807
dc.description.abstract Two bacterial consortia were enriched from uncontaminated soil by virtue of their ability to grow on 1,4-dioxane (dioxane) as a sole carbon and energy source. Their specific dioxane degradation rates at 30°C, pH = 7 (i.e. 5.7 to 7.1 g-dioxane per g-protein per day) were comparable to those of two dioxane-metabolizing archetypes: Pseudonocardia dioxanivoransCB1190 and Mycobacterium dioxanotrophicusPH-06. Based on 16S rRNA sequencing, Mycobacterium was the dominant genus. Acetylene inhibition tests suggest that dioxane degradation was mediated by monooxygenases. However, qPCR analyses targeting the tetrahydrofuran/dioxane monooxygenase gene (thmA/dxmA) (which is, to date, the only sequenced dioxane monooxygenase gene) were negative, indicating that other (as yet unknown) catabolic gene(s) were responsible. DNA sequence analyses also showed threefold to sevenfold enrichment of group 5 and group 6 soluble di-iron monooxygenase (SDIMO) genes relative to the original soil samples. Whereas biodegradation of trace levels of dioxane is a common challenge at contaminated sites, both consortia degraded dioxane at low initial concentrations (300 μg l−1) below detectable levels (5 μg l−1) in bioaugmented microcosms prepared with impacted groundwater. Overall, this work shows that dioxane-degrading bacteria (and the associated natural attenuation potential) exist even in some uncontaminated soils, and may be enriched to broaden bioaugmentation options for sites experiencing insufficient dioxane catabolic capacity.
dc.language.iso eng
dc.publisher Wiley
dc.rights This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.title 1,4-Dioxane-degrading consortia can be enriched from uncontaminated soils: prevalence of Mycobacterium and soluble di-iron monooxygenase genes
dc.type Journal article
dc.citation.journalTitle Microbial Biotechnology
dc.identifier.digital He_et_al-2017-Microbial_Biotechnology
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1111/1751-7915.12850
dc.identifier.pmid 28984418
dc.type.publication publisher version


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