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dc.contributor.authorVo, Tiffany N.
Tabata, Yasuhiko
Mikos, Antonios G.
dc.date.accessioned 2017-08-09T17:13:26Z
dc.date.available 2017-08-09T17:13:26Z
dc.date.issued 2016
dc.identifier.citation Vo, Tiffany N., Tabata, Yasuhiko and Mikos, Antonios G.. "Effects of cellular parameters on the in vitro osteogenic potential of dual-gelling mesenchymal stem cell-laden hydrogels." Journal of Biomaterials Science, Polymer Edition, 27, no. 12 (2016) Taylor & Francis: 1277-1290. http://dx.doi.org/10.1080/09205063.2016.1195157.
dc.identifier.urihttps://hdl.handle.net/1911/96633
dc.description.abstract This work investigated the effects of cellular encapsulation density and differentiation stage on the osteogenic capacity of injectable, dual physically and chemically gelling hydrogels comprised of thermogelling macromers and polyamidoamine crosslinkers. Undifferentiated and osteogenically predifferentiated mesenchymal stem cells (MSCs) were encapsulated within 20 wt% composite hydrogels with gelatin microparticles at densities of six or 15 million cells/mL. We hypothesized that a high encapsulation density and predifferentiation would promote increased cellular interaction and accelerate osteogenesis, leading to enhanced osteogenic potential in vitro. Hydrogels were able to maintain the viability of the encapsulated cells over a period of 28 days, with the high encapsulation density and predifferentiation group possessing the highest DNA content at all time points. Early alkaline phosphatase activity and mineralization were promoted by encapsulation density, whereas this effect by predifferentiation was only observed in the low seeding density groups. Both parameters only demonstrated short-lived effects when examined independently, but jointly led to greater levels of alkaline phosphatase activity and mineralization. The combined effects suggest that there may be optimal encapsulation densities and differentiation periods that need to be investigated to improve MSCs for biomaterial-based therapeutics in bone tissue engineering.
dc.language.iso eng
dc.publisher Taylor & Francis
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Taylor & Francis.
dc.title Effects of cellular parameters on the in vitro osteogenic potential of dual-gelling mesenchymal stem cell-laden hydrogels
dc.type Journal article
dc.citation.journalTitle Journal of Biomaterials Science, Polymer Edition
dc.subject.keywordMesenchymal stem cell
N-isopropylacrylamide
bone tissue engineering
predifferentiation
dc.citation.volumeNumber 27
dc.citation.issueNumber 12
dc.identifier.digital Effects_Cellular_Parameters
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1080/09205063.2016.1195157
dc.identifier.pmcid PMC4961735
dc.identifier.pmid 27328947
dc.type.publication post-print
dc.citation.firstpage 1277
dc.citation.lastpage 1290


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