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dc.contributor.authorLu, Steven
Lam, Johnny
Trachtenberg, Jordan E.
Lee, Esther J.
Seyednejad, Hajar
van den Beucken, Jeroen J.J.P.
Tabata, Yasuhiko
Wong, Mark E.
Jansen, John A.
Mikos, Antonios G.
Kasper, F. Kurtis
dc.date.accessioned 2017-08-04T12:29:41Z
dc.date.available 2017-08-04T12:29:41Z
dc.date.issued 2014
dc.identifier.citation Lu, Steven, Lam, Johnny, Trachtenberg, Jordan E., et al.. "Dual growth factor delivery from bilayered, biodegradable hydrogel composites for spatially-guided osteochondral tissue repair." Biomaterials, 35, no. 31 (2014) Elsevier: 8829-8839. https://doi.org/10.1016/j.biomaterials.2014.07.006.
dc.identifier.urihttps://hdl.handle.net/1911/96578
dc.description.abstract The present work investigated the use of biodegradable hydrogel composite scaffolds, based on the macromer oligo(poly(ethylene glycol) fumarate) (OPF), to deliver growth factors for the repair of osteochondral tissue in a rabbit model. In particular, bilayered OPF composites were used to mimic the structural layers of the osteochondral unit, and insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2) were loaded into gelatin microparticles and embedded within the OPF hydrogel matrix in a spatially controlled manner. Three different scaffold formulations were implanted in a medial femoral condyle osteochondral defect: 1) IGF-1 in the chondral layer, 2) BMP-2 in the subchondral layer, and 3) IGF-1 and BMP-2 in their respective separate layers. The quantity and quality of osteochondral repair was evaluated at 6 and 12 weeks with histological scoring and micro-computed tomography (micro-CT). While histological scoring results at 6 weeks showed no differences between experimental groups, micro-CT analysis revealed that the delivery of BMP-2 alone increased the number of bony trabecular islets formed, an indication of early bone formation, over that of IGF-1 delivery alone. At 12 weeks post-implantation, minimal differences were detected between the three groups for cartilage repair. However, the dual delivery of IGF-1 and BMP-2 had a higher proportion of subchondral bone repair, greater bone growth at the defect margins, and lower bone specific surface than the single delivery of IGF-1. These results suggest that the delivery of BMP-2 enhances subchondral bone formation and that, while the dual delivery of IGF-1 and BMP-2 in separate layers does not improve cartilage repair under the conditions studied, they may synergistically enhance the degree of subchondral bone formation. Overall, bilayered OPF hydrogel composites demonstrate potential as spatially-guided, multiple growth factor release vehicles for osteochondral tissue repair.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Dual growth factor delivery from bilayered, biodegradable hydrogel composites for spatially-guided osteochondral tissue repair
dc.type Journal article
dc.citation.journalTitle Biomaterials
dc.subject.keywordBone morphogenetic protein-2
Cartilage repair
Insulin-like growth factor-1
Rabbit model
Subchondral bone
dc.citation.volumeNumber 35
dc.citation.issueNumber 31
dc.identifier.digital spatially-guided_osteochondral_tissue_repair
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1016/j.biomaterials.2014.07.006
dc.identifier.pmcid PMC4140660
dc.identifier.pmid 25047629
dc.type.publication post-print
dc.citation.firstpage 8829
dc.citation.lastpage 8839


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