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dc.contributor.authorOhata, Jun
Farrukh, Vohidov
Ball, Zachary T.
dc.date.accessioned 2017-06-14T18:46:24Z
dc.date.available 2017-06-14T18:46:24Z
dc.date.issued 2015
dc.identifier.citation Ohata, Jun, Farrukh, Vohidov and Ball, Zachary T.. "Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents." Molecular BioSystems, 11, (2015) Royal Society of Chemistry: 2846-2849. https://doi.org/10.1039/C5MB00510H.
dc.identifier.urihttps://hdl.handle.net/1911/94851
dc.description.abstract Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide–alkyne cycloaddition conducted on the surface of a blot membrane. The method eliminates the need for separation steps to remove excess small molecule reagents before attachment of antigen molecules or other visualization handles, and is especially useful for the analysis of peptides and small proteins. A variety of potential fluorogenic reagents are assessed, and sensitivity (<0.1 picomole) similar to current commercially available fluorescence imaging methods is possible.
dc.language.iso eng
dc.publisher Royal Society of Chemistry
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Royal Society of Chemistry.
dc.title Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents
dc.type Journal article
dc.citation.journalTitle Molecular BioSystems
dc.citation.volumeNumber 11
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1039/C5MB00510H
dc.identifier.pmcid PMC4605879
dc.identifier.pmid 26325302
dc.type.publication post-print
dc.citation.firstpage 2846
dc.citation.lastpage 2849


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