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dc.contributor.authorFloriano, Pierre
Abram, Tim
Taylor, Leander
Le, Cathy
Talavera, Humberto
Nguyen, Michael
Raja, Rameez
Gillenwater, Ann
McDevitt, John
Vigneswaran, Nadarajah
dc.date.accessioned 2017-06-14T16:55:02Z
dc.date.available 2017-06-14T16:55:02Z
dc.date.issued 2015
dc.identifier.citation Floriano, Pierre, Abram, Tim, Taylor, Leander, et al.. "Programmable bio-nanochip-based cytologic testing of oral potentially malignant disorders in Fanconi anemia." Oral Diseases, 21, no. 5 (2015) Wiley: 593-601. https://doi.org/10.1111/odi.12321.
dc.identifier.urihttps://hdl.handle.net/1911/94843
dc.description.abstract Fanconi anemia (FA) is caused by mutations of DNA repair genes. The risk of oral squamous cell carcinoma (OSCC) among FA patients is 800-folds higher than in the general population. Early detection of OSCC, preferably at it precursor stage, is critical in FA patients to improve their survival. In an ongoing clinical trial, we are evaluating the effectiveness of the programmable bio-nanochip (p-BNC)-based oral cytology test in diagnosing oral potentially malignant disorders (OPMD) in non-FA patients. We used this test to compare cytomorphometric and molecular biomarkers in OSCC cell lines derived from FA and non-FA patients to brush biopsy samples of a FA patient with OPMD and normal mucosa of healthy volunteers. Our data showed that expression patterns of molecular biomarkers were not notably different between sporadic and FA-OSCC cell lines. The p-BNC assay revealed significant differences in cytometric parameters and biomarker MCM2 expression between cytobrush samples of the FA patient and cytobrush samples of normal oral mucosa obtained from healthy volunteers. Microscopic examination of the FA patient's OPMD confirmed the presence of dysplasia. Our pilot data suggests that the p-BNC brush biopsy test recognized dysplastic oral epithelial cells in a brush biopsy sample of a FA patient.
dc.language.iso eng
dc.publisher Wiley
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
dc.title Programmable bio-nanochip-based cytologic testing of oral potentially malignant disorders in Fanconi anemia
dc.type Journal article
dc.citation.journalTitle Oral Diseases
dc.subject.keywordFanconi anemia
bio-nanochip
brush biopsy
oral epithelial dysplasia
oral potentially malignant disorders
oral squamous cell carcinoma
dc.citation.volumeNumber 21
dc.citation.issueNumber 5
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1111/odi.12321
dc.identifier.pmcid PMC4457567
dc.identifier.pmid 25662766
dc.type.publication post-print
dc.citation.firstpage 593
dc.citation.lastpage 601


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