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dc.contributor.authorLam, Johnny
Kim, Kyobum
Lu, Steven
Tabata, Yasuhiko
Scott, David W.
Mikos, Antonios G.
Kasper, F. Kurtis
dc.date.accessioned 2017-06-14T16:55:02Z
dc.date.available 2017-06-14T16:55:02Z
dc.date.issued 2014
dc.identifier.citation Lam, Johnny, Kim, Kyobum, Lu, Steven, et al., . "A factorial analysis of the combined effects of hydrogel fabrication parameters on the in vitro swelling and degradation of oligo(poly(ethylene glycol) fumarate) hydrogels." Journal of Biomedical Materials Research, 102, no. 10 (2014) 3477-3487. https://doi.org/10.1002/jbm.a.35015.
dc.identifier.urihttp://hdl.handle.net/1911/94835
dc.description.abstract In this study, a full factorial approach was used to investigate the effects of poly(ethylene glycol) (PEG) molecular weight (MW; 10,000 vs. 35,000 nominal MW), crosslinker-to-macromer carbon–carbon double bond ratio (DBR; 40 vs. 60), crosslinker type (PEG-diacrylate (PEGDA) vs. N,N′-methylene bisacrylamide (MB)), crosslinking extent of incorporated gelatin microparticles (low vs. high), and incubation medium composition (with or without collagenase) on the swelling and degradation characteristics of oligo[(poly(ethylene glycol) fumarate)] (OPF) hydrogel composites as indicated by the swelling ratio and the percentage of mass remaining, respectively. Each factor consisted of two levels, which were selected based on previous in vitro and in vivo studies utilizing these hydrogels for various tissue engineering applications. Fractional factorial analyses of the main effects indicated that the mean swelling ratio and the mean percentage of mass remaining of OPF composite hydrogels were significantly affected by every factor. In particular, increasing the PEG chain MW of OPF macromers significantly increased the mean swelling ratio and decreased the mean percentage of mass remaining by 5.7 ± 0.3 and 17.2 ± 0.6%, respectively. However, changing the crosslinker from MB to PEGDA reduced the mean swelling ratio and increased the mean percentage of mass remaining of OPF composite hydrogels by 4.9 ± 0.2 and 9.4 ± 0.9%, respectively. Additionally, it was found that the swelling characteristics of hydrogels fabricated with higher PEG chain MW or with MB were more sensitive to increases in DBR. Collectively, the main and cross effects observed between factors enables informed tuning of the swelling and degradation properties of OPF-based hydrogels for various tissue engineering applications.
dc.language.iso eng
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
dc.title A factorial analysis of the combined effects of hydrogel fabrication parameters on the in vitro swelling and degradation of oligo(poly(ethylene glycol) fumarate) hydrogels
dc.type Journal article
dc.citation.journalTitle Journal of Biomedical Materials Research
dc.subject.keywordfabrication parameters
factorial study
hydrogels
poly(ethylene glycol)-based materials
swelling and degradation
dc.citation.volumeNumber 102
dc.citation.issueNumber 10
dc.contributor.publisher Wiley
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1002/jbm.a.35015
dc.identifier.pmcid PMC4012001
dc.identifier.pmid 24243766
dc.type.publication post-print
dc.citation.firstpage 3477
dc.citation.lastpage 3487


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