Show simple item record

dc.contributor.authorDahlin, Rebecca L.
Kinard, Lucas A.
Lam, Johnny
Needham, Clark J.
Lu, Steven
Kasper, F. Kurtis
Mikos, Antonios G.
dc.date.accessioned 2017-06-12T21:49:43Z
dc.date.available 2017-06-12T21:49:43Z
dc.date.issued 2014
dc.identifier.citation Dahlin, Rebecca L., Kinard, Lucas A., Lam, Johnny, et al.. "Articular chondrocytes and mesenchymal stem cells seeded on biodegradable scaffolds for the repair of cartilage in a rat osteochondral defect model." Biomaterials, 35, no. 26 (2014) Elsevier: 7460-7469. https://doi.org/10.1016/j.biomaterials.2014.05.055.
dc.identifier.urihttps://hdl.handle.net/1911/94831
dc.description.abstract This work investigated the ability of co-cultures of articular chondrocytes and mesenchymal stem cells (MSCs) to repair articular cartilage in osteochondral defects. Bovine articular chondrocytes and rat MSCs were seeded in isolation or in co-culture onto electrospun poly(ɛ-caprolactone) (PCL) scaffolds and implanted into an osteochondral defect in the trochlear groove of 12-week old Lewis rats. Additionally, a blank PCL scaffold and untreated defect were investigated. After 12 weeks, the extent of cartilage repair was analyzed through histological analysis, and the extent of bone healing was assessed by quantifying the total volume of mineralized bone in the defect through microcomputed tomography. Histological analysis revealed that the articular chondrocytes and co-cultures led to repair tissue that consisted of more hyaline-like cartilage tissue that was thicker and possessed more intense Safranin O staining. The MSC, blank PCL scaffold, and empty treatment groups generally led to the formation of fibrocartilage repair tissue. Microcomputed tomography revealed that while there was an equivalent amount of mineralized bone formation in the MSC, blank PCL, and empty treatment groups, the defects treated with chondrocytes or co-cultures had negligible mineralized bone formation. Overall, even with a reduced number of chondrocytes, co-cultures led to an equal level of cartilage repair compared to the chondrocyte samples, thus demonstrating the potential for the use of co-cultures of articular chondrocytes and MSCs for the in vivo repair of cartilage defects.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Articular chondrocytes and mesenchymal stem cells seeded on biodegradable scaffolds for the repair of cartilage in a rat osteochondral defect model
dc.type Journal article
dc.citation.journalTitle Biomaterials
dc.subject.keywordArticular chondrocyte
Mesenchymal stem cell
Co-culture
Rat osteochondral defect
Cartilage regeneration
dc.citation.volumeNumber 35
dc.citation.issueNumber 26
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1016/j.biomaterials.2014.05.055
dc.identifier.pmcid PMC4109803
dc.identifier.pmid 24927682
dc.type.publication post-print
dc.citation.firstpage 7460
dc.citation.lastpage 7469


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record