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dc.contributor.authorAlmeida, Joao Paulo Mattos
Lin, Adam Yuh
Langsner, Robert James
Eckels, Phillip
Foster, Aaron Edward
Drezek, Rebekah Anna
dc.date.accessioned 2017-06-06T19:07:23Z
dc.date.available 2017-06-06T19:07:23Z
dc.date.issued 2014
dc.identifier.citation Almeida, Joao Paulo Mattos, Lin, Adam Yuh, Langsner, Robert James, et al.. "In Vivo Immune Cell Distribution of Gold Nanoparticles in Naïve and Tumor Bearing Mice." Small, 10, no. 4 (2014) Wiley: 812-819. https://doi.org/10.1002/smll.201301998.
dc.identifier.urihttps://hdl.handle.net/1911/94813
dc.description.abstract Gold nanoparticles (AuNP) have been widely used for drug delivery and have recently been explored for applications in cancer immunotherapy. Although AuNPs are known to accumulate heavily in the spleen, the particle distribution within immune cells has not been thoroughly studied. Here, cellular distribution of Cy5 labeled 50 nm AuNPs is characterized within the immune populations of the spleen from naïve and tumor bearing mice using flow cytometry. Surprisingly, approximately 30% of the detected AuNPs are taken up by B cells at 24 h, with about 10% in granulocytes, 18% in dendritic cells, and 8% in T cells. In addition, 3% of the particles are detected within myeloid derived suppressor cells, an immune suppressive population that could be targeted for cancer immunotherapy. Furthermore, it is observed that, over time, the particles traveled from the red pulp and marginal zone to the follicles of the spleen. Taking into consideration that the particle cellular distribution does not change at 1, 6 and 24 h, it is highly suggestive that the immune populations carry the particles and migrate through the spleen instead of the particles migrating through the tissue by cell-cell transfer. Finally, no difference is observed in particle distribution between naïve and tumor bearing mice in the spleen, and nanoparticles are detected within 0.7% of dendritic cells of the tumor microenvironment. Overall, these results can help inform and influence future AuNP delivery design criteria including future applications for nanoparticle-mediated immunotherapy.
dc.language.iso eng
dc.publisher Wiley
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
dc.title In Vivo Immune Cell Distribution of Gold Nanoparticles in Naïve and Tumor Bearing Mice
dc.type Journal article
dc.citation.journalTitle Small
dc.subject.keywordbiodistribution
cancer
gold nanoparticles
immune system
immunotherapy
spleen
dc.citation.volumeNumber 10
dc.citation.issueNumber 4
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1002/smll.201301998
dc.identifier.pmcid PMC3962812
dc.identifier.pmid 24115675
dc.type.publication post-print
dc.citation.firstpage 812
dc.citation.lastpage 819


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