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    In Vivo Immune Cell Distribution of Gold Nanoparticles in Naïve and Tumor Bearing Mice

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    Author
    Almeida, Joao Paulo Mattos; Lin, Adam Yuh; Langsner, Robert James; Eckels, Phillip; Foster, Aaron Edward; More... Drezek, Rebekah Anna Less...
    Date
    2014
    Abstract
    Gold nanoparticles (AuNP) have been widely used for drug delivery and have recently been explored for applications in cancer immunotherapy. Although AuNPs are known to accumulate heavily in the spleen, the particle distribution within immune cells has not been thoroughly studied. Here, cellular distribution of Cy5 labeled 50 nm AuNPs is characterized within the immune populations of the spleen from naïve and tumor bearing mice using flow cytometry. Surprisingly, approximately 30% of the detected AuNPs are taken up by B cells at 24 h, with about 10% in granulocytes, 18% in dendritic cells, and 8% in T cells. In addition, 3% of the particles are detected within myeloid derived suppressor cells, an immune suppressive population that could be targeted for cancer immunotherapy. Furthermore, it is observed that, over time, the particles traveled from the red pulp and marginal zone to the follicles of the spleen. Taking into consideration that the particle cellular distribution does not change at 1, 6 and 24 h, it is highly suggestive that the immune populations carry the particles and migrate through the spleen instead of the particles migrating through the tissue by cell-cell transfer. Finally, no difference is observed in particle distribution between naïve and tumor bearing mice in the spleen, and nanoparticles are detected within 0.7% of dendritic cells of the tumor microenvironment. Overall, these results can help inform and influence future AuNP delivery design criteria including future applications for nanoparticle-mediated immunotherapy.
    Citation
    Almeida, Joao Paulo Mattos, Lin, Adam Yuh, Langsner, Robert James, et al.. "In Vivo Immune Cell Distribution of Gold Nanoparticles in Naïve and Tumor Bearing Mice." Small, 10, no. 4 (2014) Wiley: 812-819. https://doi.org/10.1002/smll.201301998.
    Published Version
    https://doi.org/10.1002/smll.201301998
    Keyword
    biodistribution; cancer; gold nanoparticles; immune system; immunotherapy; More... spleen Less...
    Type
    Journal article
    Publisher
    Wiley
    Citable link to this page
    https://hdl.handle.net/1911/94813
    Rights
    This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
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    • Bioengineering Publications [684]
    • Faculty Publications [5504]

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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map