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dc.contributor.authorPerl, Daniel P.
Parikh, Neil
Chang, Shannon
Peng, Paul
Thekkek, Nadhi
Lee, Michelle H.
Polydorides, Alexandros D.
Mitcham, Josephine
Richards-Kortum, Rebecca
Anandasabapathy, Sharmila
dc.date.accessioned 2017-06-05T20:44:20Z
dc.date.available 2017-06-05T20:44:20Z
dc.date.issued 2014
dc.identifier.citation Perl, Daniel P., Parikh, Neil, Chang, Shannon, et al.. "Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging." Journal of Visualized Experiments, 87, (2014) JoVE: https://doi.org/10.3791/50992.
dc.identifier.urihttps://hdl.handle.net/1911/94800
dc.description.abstract The ability to differentiate benign metaplasia in Barrett’s Esophagus (BE) from neoplasia in vivo remains difficult as both tissue types can be flat and indistinguishable with white light imaging alone. As a result, a modality that highlights glandular architecture would be useful to discriminate neoplasia from benign epithelium in the distal esophagus. VFI is a novel technique that uses an exogenous topical fluorescent contrast agent to delineate high grade dysplasia and cancer from benign epithelium. Specifically, the fluorescent images provide spatial resolution of 50 to 100 μm and a field of view up to 2.5 cm, allowing endoscopists to visualize glandular morphology. Upon excitation, classic Barrett’s metaplasia appears as continuous, evenly-spaced glands and an overall homogenous morphology; in contrast, neoplastic tissue appears crowded with complete obliteration of the glandular framework. Here we provide an overview of the instrumentation and enumerate the protocol of this new technique. While VFI affords a gastroenterologist with the glandular architecture of suspicious tissue, cellular dysplasia cannot be resolved with this modality. As such, one cannot morphologically distinguish Barrett’s metaplasia from BE with Low-Grade Dysplasia via this imaging modality. By trading off a decrease in resolution with a greater field of view, this imaging system can be used at the very least as a red-flag imaging device to target and biopsy suspicious lesions; yet, if the accuracy measures are promising, VFI may become the standard imaging technique for the diagnosis of neoplasia (defined as either high grade dysplasia or cancer) in the distal esophagus.
dc.language.iso eng
dc.publisher JoVE
dc.rights Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.title Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging
dc.type Journal article
dc.citation.journalTitle Journal of Visualized Experiments
dc.citation.volumeNumber 87
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.3791/50992
dc.identifier.pmcid PMC4089432
dc.identifier.pmid 24893592
dc.type.publication publisher version


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