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dc.contributor.authorChristodoulides, Nicolaos
De La Garza, Richard II
Simmons, Glennon W.
McRae, Michael P.
Wong, Jorge
Newton, Thomas F.
Smith, Regina
Mahoney, James J. III
Hohenstein, Justin
Gomez, Sobeyda
Floriano, Pierre N.
Talavera, Humberto
Sloan, Daniel J.
Moody, David E.
Andrenyak, David M.
Kosten, Thomas R.
Haque, Ahmed
McDevitt, John T.
dc.date.accessioned 2017-05-30T17:03:41Z
dc.date.available 2017-05-30T17:03:41Z
dc.date.issued 2015
dc.identifier.citation Christodoulides, Nicolaos, De La Garza, Richard II, Simmons, Glennon W., et al.. "Application of programmable bio-nano-chip system for the quantitative detection of drugs of abuse in oral fluids." Drug and Alcohol Dependence, 153, (2015) 306-313. http://dx.doi.org/10.1016/j.drugalcdep.2015.04.026.
dc.identifier.urihttps://hdl.handle.net/1911/94741
dc.description.abstract Objective: There is currently a gap in on-site drug of abuse monitoring. Current detection methods involve invasive sampling of blood and urine specimens, or collection of oral fluid, followed by qualitative screening tests using immunochromatographic cartridges. While remote laboratories then may provide confirmation and quantitative assessment of a presumptive positive, this instrumentation is expensive and decoupled from the initial sampling making the current drug-screening program inefficient and costly. The authors applied a noninvasive oral fluid sampling approach integrated with the in-development chip-based Programmable bio-nano-chip (p-BNC) platform for the detection of drugs of abuse. Method: The p-BNC assay methodology was applied for the detection of tetrahydrocannabinol, morphine, amphetamine, methamphetamine, cocaine, methadone and benzodiazepines, initially using spiked buffered samples and, ultimately, using oral fluid specimen collected from consented volunteers. Results: Rapid (∼10 min), sensitive detection (∼ng/mL) and quantitation of 12 drugs of abuse was demonstrated on the p-BNC platform. Furthermore, the system provided visibility to time-course of select drug and metabolite profiles in oral fluids; for the drug cocaine, three regions of slope were observed that, when combined with concentration measurements from this and prior impairment studies, information about cocaine-induced impairment may be revealed. Conclusions: This chip-based p-BNC detection modality has significant potential to be used in the future by law enforcement officers for roadside drug testing and to serve a variety of other settings, including outpatient and inpatient drug rehabilitation centers, emergency rooms, prisons, schools, and in the workplace.
dc.language.iso eng
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Application of programmable bio-nano-chip system for the quantitative detection of drugs of abuse in oral fluids
dc.type Journal article
dc.citation.journalTitle Drug and Alcohol Dependence
dc.subject.keyworddrugs of abuse
programmable bio-nano-chip
oral fluid
multiplexed
on-site testing
dc.citation.volumeNumber 153
dc.contributor.publisher Elsevier
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.drugalcdep.2015.04.026
dc.identifier.pmcid PMC4509839
dc.identifier.pmid 26048639
dc.type.publication post-print
dc.citation.firstpage 306
dc.citation.lastpage 313


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