Show simple item record

dc.contributor.authorHan, Lu
Singh, Shanteri
Thorson, Jon S.
Phillips, George N. Jr.
dc.date.accessioned 2017-05-22T21:48:43Z
dc.date.available 2017-05-22T21:48:43Z
dc.date.issued 2016
dc.identifier.citation Han, Lu, Singh, Shanteri, Thorson, Jon S., et al.. "Loop dynamics of thymidine diphosphate-rhamnose 3′-O-methyltransferase (CalS11), an enzyme in calicheamicin biosynthesis." Structural Dynamics, 3, no. 1 (2016) AIP Publishing: http://dx.doi.org/10.1063/1.4941368.
dc.identifier.urihttps://hdl.handle.net/1911/94348
dc.description.abstract Structure analysis and ensemble refinement of the apo-structure of thymidine diphosphate (TDP)-rhamnose 3′-O-methyltransferase reveal a gate for substrate entry and product release. TDP-rhamnose 3′-O-methyltransferase (CalS11) catalyses a 3′-O-methylation of TDP-rhamnose, an intermediate in the biosynthesis of enediyne antitumor antibiotic calicheamicin. CalS11 operates at the sugar nucleotide stage prior to glycosylation step. Here, we present thecrystal structure of the apo form of CalS11 at 1.89 Å resolution. We propose that the L2 loop functions as a gate facilitating and/or providing specificity for substrate entry or promoting product release. Ensemble refinement analysis slightly improves the crystallographic refinement statistics and furthermore provides a compelling way to visualize the dynamic model of loop L2, supporting the understanding of its proposed role in catalysis.
dc.language.iso eng
dc.publisher AIP Publishing
dc.rights Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.title Loop dynamics of thymidine diphosphate-rhamnose 3′-O-methyltransferase (CalS11), an enzyme in calicheamicin biosynthesis
dc.type Journal article
dc.citation.journalTitle Structural Dynamics
dc.citation.volumeNumber 3
dc.citation.issueNumber 1
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1063/1.4941368
dc.identifier.pmcid PMC4760980
dc.identifier.pmid 26958582
dc.type.publication publisher version
dc.citation.articleNumber 012004


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record