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dc.contributor.authorWong, Cynthia
Ha, Ngan P.
Pawlowski, Michal E.
Graviss, Edward A.
Tkaczyk, Tomasz S.
dc.date.accessioned 2017-05-22T17:16:48Z
dc.date.available 2017-05-22T17:16:48Z
dc.date.issued 2016
dc.identifier.citation Wong, Cynthia, Ha, Ngan P., Pawlowski, Michal E., et al.. "Differentiating between live and deadᅠMycobacterium smegmatisᅠusing autofluorescence." Tuberculosis, 101, (2016) Elsevier: S119-S123. https://doi.org/10.1016/j.tube.2016.09.010.
dc.identifier.urihttps://hdl.handle.net/1911/94315
dc.description.abstract While there have been research efforts to find faster and more efficient diagnostic techniques for tuberculosis (TB), it is equally important to monitor a patient's response to treatment over time, especially with the increasing prevalence of multi-drug resistant (MDR) and extensively-drug resistant (XDR) TB. Between sputum smear microscopy, culture, and GeneXpert, only culture can verify viability of mycobacteria. However, it may take up to six weeks to grow Mycobacterium tuberculosis (Mtb), during which time the patient may have responded to treatment or the mycobacteria are still viable because the patient has MDR or XDR TB. In both situations, treatment incurs increased patient costs and makes them more susceptible to host-drug effects such as liver damage. Coenzyme Factor 420 (F420) is a fluorescent coenzyme found naturally in mycobacteria, with an excitation peak around 420 nm and an emission peak around 470 nm. Using Mycobacterium smegmatis, we show that live and dead mycobacteria undergo different rates of photobleaching over a period of 2 min. These preliminary experiments suggest that the different photobleaching rates could be used to help monitor a patient's response to TB treatment. In future studies, we propose to describe these experiments with Mtb as both M. smegmatis and Mtb use F420.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Differentiating between live and deadᅠMycobacterium smegmatisᅠusing autofluorescence
dc.type Journal article
dc.citation.journalTitle Tuberculosis
dc.subject.keywordAutofluorescence
Microscopy
Mycobacterium
Viability
dc.citation.volumeNumber 101
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1016/j.tube.2016.09.010
dc.identifier.pmcid PMC5183484
dc.identifier.pmid 27742463
dc.type.publication post-print
dc.citation.firstpage S119
dc.citation.lastpage S123


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