Show simple item record

dc.contributor.authorHan, Richard I.
Wheeler, Thomas M.
Lumsden, Alan B.
Reardon, Michael J.
Lawrie, Gerald M.
Grande-Allen, K. Jane
Morrisett, Joel D.
Brunner, Gerd
dc.date.accessioned 2017-05-09T20:16:07Z
dc.date.available 2017-05-09T20:16:07Z
dc.date.issued 2016
dc.identifier.citation Han, Richard I., Wheeler, Thomas M., Lumsden, Alan B., et al.. "Morphometric analysis of calcification and fibrous layer thickness in carotid endarterectomy tissues." Computers in Biology and Medicine, 70, (2016) 210-219. https://doi.org/10.1016/j.compbiomed.2016.01.019.
dc.identifier.urihttps://hdl.handle.net/1911/94217
dc.description.abstract Background: Advanced atherosclerotic lesions are commonly characterized by the presence of calcification. Several studies indicate that extensive calcification is associated with plaque stability, yet recent studies suggest that calcification morphology and location may adversely affect the mechanical stability of atherosclerotic plaques. The underlying cause of atherosclerotic calcification and the importance of intra-plaque calcium distribution remains poorly understood. Method: The goal of this study was the characterization of calcification morphology based on histological features in 20 human carotid endarterectomy (CEA) specimens. Representative frozen sections (10 μm thick) were cut from the common, bulb, internal and external segments of CEA tissues and stained with von Kossa׳s reagent for calcium phosphate. The morphology of calcification (calcified patches) and fibrous layer thickness were quantified in 135 histological sections. Results: Intra-plaque calcification was distributed heterogeneously (calcification %-area: bulb segment: 14.2±2.1%; internal segment: 12.9±2.8%; common segment: 4.6±1.1%; p=0.001). Calcified patches were found in 20 CEAs (patch size: <0.1mm2 to >1.0mm2). Calcified patches were most abundant in the bulb and least in the common segment (bulb n=7.30±1.08; internal n=4.81±1.17; common n=2.56±0.56; p=0.0007). Calcified patch circularity decreased with increasing size (<0.1 mm2: 0.77±0.01, 0.1–1 mm2: 0.62±0.01, >1.0 mm2: 0.51±0.02; p=0.0001). A reduced fibrous layer thickness was associated with increased calcium patch size (p<0.0001). Conclusions: In advanced carotid atherosclerosis, calcification appears to be a heterogeneous and dynamic atherosclerotic plaque component, as indicated by the simultaneous presence of few large stabilizing calcified patches and numerous small calcific patches. Future studies are needed to elucidate the associations of intra-plaque calcification size and distribution with atherothrombotic events.
dc.language.iso eng
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Morphometric analysis of calcification and fibrous layer thickness in carotid endarterectomy tissues
dc.type Journal article
dc.citation.journalTitle Computers in Biology and Medicine
dc.subject.keywordcarotid endarterectomy
calcification
image analysis
carotid bifurcation
fibrous layer
dc.citation.volumeNumber 70
dc.contributor.publisher Elsevier
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1016/j.compbiomed.2016.01.019
dc.identifier.pmcid PMC4765924
dc.identifier.pmid 26851729
dc.type.publication post-print
dc.citation.firstpage 210
dc.citation.lastpage 219


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record