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dc.contributor.authorBlancas, Alicia A.
Balaoing, Liezl R.
Acosta, Francisca M.
Grande-Allen, K. Jane
dc.date.accessioned 2017-05-05T19:00:54Z
dc.date.available 2017-05-05T19:00:54Z
dc.date.issued 2016
dc.identifier.citation Blancas, Alicia A., Balaoing, Liezl R., Acosta, Francisca M., et al.. "Identifying Behavioral Phenotypes and Heterogeneity in Heart Valve Surface Endothelium." Cells Tissues Organs, 201, no. 4 (2016) Karger: 268-276. https://doi.org/10.1159/000444446.
dc.identifier.urihttps://hdl.handle.net/1911/94201
dc.description.abstract Heart valvular endothelial cells (VECs) are distinct from vascular endothelial cells (ECs), but have an uncertain context within the spectrum of known endothelial phenotypes, including lymphatic ECs (LECs). Profiling the phenotypes of the heart valve surface VECs would facilitate identification of a proper seeding population for tissue-engineered valves, as well as elucidate mechanisms of valvular disease. Porcine VECs and porcine aortic ECs (AECs) were isolated from pig hearts and characterized to assess known EC and LEC markers. A transwell migration assay determined their propensity to migrate toward vascular endothelial growth factor, an angiogenic stimulus, over 24 h. Compared to AECs, Flt-1 was expressed on almost double the percentage of VECs, measured as 74 versus 38%. The expression of angiogenic EC markers CXCR4 and DLL4 was >90% on AECs, whereas VECs showed only 35% CXCR4+ and 47% DLL4+. AECs demonstrated greater migration (71.5 ± 11.0 cells per image field) than the VECs with 30.0 ± 15.3 cells per image field (p = 0.032). In total, 30% of VECs were positive for LYVE1+/Prox1+, while these markers were absent in AECs. In conclusion, the population of cells on the surface of heart valves is heterogeneous, consisting largely of nonangiogenic VECs and a subset of LECs. Previous studies have indicated the presence of LECs within the interior of the valves; however, this is the first study to demonstrate their presence on the surface. Identification of this unique endothelial mixture is a step forward in the development of engineered valve replacements as a uniform EC seeding population may not be the best option to maximize transplant success.
dc.language.iso eng
dc.publisher Karger
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Karger.
dc.title Identifying Behavioral Phenotypes and Heterogeneity in Heart Valve Surface Endothelium
dc.type Journal article
dc.citation.journalTitle Cells Tissues Organs
dc.subject.keywordendothelium
aortic valve
lymphatic
angiogenesis
dc.citation.volumeNumber 201
dc.citation.issueNumber 4
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1159/000444446
dc.identifier.pmcid PMC4874248
dc.identifier.pmid 27144771
dc.type.publication post-print
dc.citation.firstpage 268
dc.citation.lastpage 276


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