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    Identifying Behavioral Phenotypes and Heterogeneity in Heart Valve Surface Endothelium

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    Author
    Blancas, Alicia A.; Balaoing, Liezl R.; Acosta, Francisca M.; Grande-Allen, K. Jane
    Date
    2016
    Abstract
    Heart valvular endothelial cells (VECs) are distinct from vascular endothelial cells (ECs), but have an uncertain context within the spectrum of known endothelial phenotypes, including lymphatic ECs (LECs). Profiling the phenotypes of the heart valve surface VECs would facilitate identification of a proper seeding population for tissue-engineered valves, as well as elucidate mechanisms of valvular disease. Porcine VECs and porcine aortic ECs (AECs) were isolated from pig hearts and characterized to assess known EC and LEC markers. A transwell migration assay determined their propensity to migrate toward vascular endothelial growth factor, an angiogenic stimulus, over 24 h. Compared to AECs, Flt-1 was expressed on almost double the percentage of VECs, measured as 74 versus 38%. The expression of angiogenic EC markers CXCR4 and DLL4 was >90% on AECs, whereas VECs showed only 35% CXCR4+ and 47% DLL4+. AECs demonstrated greater migration (71.5 ± 11.0 cells per image field) than the VECs with 30.0 ± 15.3 cells per image field (p = 0.032). In total, 30% of VECs were positive for LYVE1+/Prox1+, while these markers were absent in AECs. In conclusion, the population of cells on the surface of heart valves is heterogeneous, consisting largely of nonangiogenic VECs and a subset of LECs. Previous studies have indicated the presence of LECs within the interior of the valves; however, this is the first study to demonstrate their presence on the surface. Identification of this unique endothelial mixture is a step forward in the development of engineered valve replacements as a uniform EC seeding population may not be the best option to maximize transplant success.
    Citation
    Blancas, Alicia A., Balaoing, Liezl R., Acosta, Francisca M., et al.. "Identifying Behavioral Phenotypes and Heterogeneity in Heart Valve Surface Endothelium." Cells Tissues Organs, 201, no. 4 (2016) Karger: 268-276. https://doi.org/10.1159/000444446.
    Published Version
    https://doi.org/10.1159/000444446
    Keyword
    endothelium; aortic valve; lymphatic; angiogenesis
    Type
    Journal article
    Publisher
    Karger
    Citable link to this page
    https://hdl.handle.net/1911/94201
    Rights
    This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Karger.
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    • Bioengineering Publications [632]
    • Faculty Publications [4990]

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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map