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dc.contributor.authorOlson, Evan J.
Tzouanas, Constantine N.
Tabor, Jeffrey J.
dc.date.accessioned 2017-05-04T18:16:35Z
dc.date.available 2017-05-04T18:16:35Z
dc.date.issued 2017
dc.identifier.citation Olson, Evan J., Tzouanas, Constantine N. and Tabor, Jeffrey J.. "A photoconversion model for full spectral programming and multiplexing of optogeneticᅠsystems." Molecular Systems Biology, 13, (2017) EMBO Press: https://doi.org/10.15252/msb.20167456.
dc.identifier.urihttps://hdl.handle.net/1911/94172
dc.description.abstract Optogenetics combines externally applied light signals and genetically engineered photoreceptors to control cellular processes with unmatched precision. Here, we develop a mathematical model of wavelength‐ and intensity‐dependent photoconversion, signaling, and output gene expression for our two previously engineered light‐sensing Escherichia coli two‐component systems. To parameterize the model, we develop a simple set of spectral and dynamical calibration experiments using our recent open‐source “Light Plate Apparatus” device. In principle, the parameterized model should predict the gene expression response to any time‐varying signal from any mixture of light sources with known spectra. We validate this capability experimentally using a suite of challenging light sources and signals very different from those used during the parameterization process. Furthermore, we use the model to compensate for significant spectral cross‐reactivity inherent to the two sensors in order to develop a new method for programming two simultaneous and independent gene expression signals within the same cell. Our optogenetic multiplexing method will enable powerful new interrogations of how metabolic, signaling, and decision‐making pathways integrate multiple input signals.
dc.language.iso eng
dc.publisher EMBO Press
dc.rights This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.title A photoconversion model for full spectral programming and multiplexing of optogeneticᅠsystems
dc.type Journal article
dc.citation.journalTitle Molecular Systems Biology
dc.subject.keywordoptogenetics
predictive modeling
synthetic biology
spectral multiplexing
two-component systems
dc.citation.volumeNumber 13
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.15252/msb.20167456
dc.identifier.pmcid PMC5408778
dc.identifier.pmid 28438832
dc.type.publication publisher version
dc.citation.articleNumber 926


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This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.