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dc.contributor.authorVo, T.N.
Shah, S.R.
Lu, S.
Tatara, A.M.
Lee, E.J.
Roh, T.T.
Tabata, Y.
Mikos, A.G.
dc.date.accessioned 2017-05-03T21:11:46Z
dc.date.available 2017-05-03T21:11:46Z
dc.date.issued 2016
dc.identifier.citation Vo, T.N., Shah, S.R., Lu, S., et al.. "Injectable dual-gelling cell-laden composite hydrogels for bone tissue engineering." Biomaterials, 83, (2016) Elsevier: 1-11. https://doi.org/10.1016/j.biomaterials.2015.12.026.
dc.identifier.urihttps://hdl.handle.net/1911/94155
dc.description.abstract The present work investigated the osteogenic potential of injectable, dual thermally and chemically gelable composite hydrogels for mesenchymal stem cell (MSC) delivery in vitro and in vivo. Composite hydrogels comprising copolymer macromers of N-isopropylacrylamide were fabricated through the incorporation of gelatin microparticles (GMPs) as enzymatically digestible porogens and sites for cellular attachment. High and low polymer content hydrogels with and without GMP loading were shown to successfully encapsulate viable MSCs and maintain their survival over 28 days in vitro. GMP incorporation was also shown to modulate alkaline phosphatase production, but enhanced hydrogel mineralization along with higher polymer content even in the absence of cells. Moreover, the regenerative capacity of 2 mm thick hydrogels with GMPs only, MSCs only, or GMPs and MSCs was evaluated in vivo in an 8 mm rat critical size cranial defect for 4 and 12 weeks. GMP incorporation led to enhanced bony bridging and mineralization within the defect at each timepoint, and direct bone-implant contact as determined by microcomputed tomography and histological scoring, respectively. Encapsulation of both GMPs and MSCs enabled hydrogel degradation leading to significant tissue infiltration and osteoid formation. The results suggest that these injectable, dual-gelling cell-laden composite hydrogels can facilitate bone ingrowth and integration, warranting further investigation for bone tissue engineering.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Injectable dual-gelling cell-laden composite hydrogels for bone tissue engineering
dc.type Journal article
dc.citation.journalTitle Biomaterials
dc.subject.keywordGelatin microparticles
N-isopropylacrylamide
Critical size cranial defect
Mesenchymal stem cells
Mineralization
dc.citation.volumeNumber 83
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1016/j.biomaterials.2015.12.026
dc.identifier.pmcid PMC4754149
dc.identifier.pmid 26773659
dc.type.publication post-print
dc.citation.firstpage 1
dc.citation.lastpage 11


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