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    Injectable dual-gelling cell-laden composite hydrogels for bone tissue engineering

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    Author
    Vo, T.N.; Shah, S.R.; Lu, S.; Tatara, A.M.; Lee, E.J.; More... Roh, T.T.; Tabata, Y.; Mikos, A.G. Less...
    Date
    2016
    Abstract
    The present work investigated the osteogenic potential of injectable, dual thermally and chemically gelable composite hydrogels for mesenchymal stem cell (MSC) delivery in vitro and in vivo. Composite hydrogels comprising copolymer macromers of N-isopropylacrylamide were fabricated through the incorporation of gelatin microparticles (GMPs) as enzymatically digestible porogens and sites for cellular attachment. High and low polymer content hydrogels with and without GMP loading were shown to successfully encapsulate viable MSCs and maintain their survival over 28 days in vitro. GMP incorporation was also shown to modulate alkaline phosphatase production, but enhanced hydrogel mineralization along with higher polymer content even in the absence of cells. Moreover, the regenerative capacity of 2 mm thick hydrogels with GMPs only, MSCs only, or GMPs and MSCs was evaluated in vivo in an 8 mm rat critical size cranial defect for 4 and 12 weeks. GMP incorporation led to enhanced bony bridging and mineralization within the defect at each timepoint, and direct bone-implant contact as determined by microcomputed tomography and histological scoring, respectively. Encapsulation of both GMPs and MSCs enabled hydrogel degradation leading to significant tissue infiltration and osteoid formation. The results suggest that these injectable, dual-gelling cell-laden composite hydrogels can facilitate bone ingrowth and integration, warranting further investigation for bone tissue engineering.
    Citation
    Vo, T.N., Shah, S.R., Lu, S., et al.. "Injectable dual-gelling cell-laden composite hydrogels for bone tissue engineering." Biomaterials, 83, (2016) Elsevier: 1-11. https://doi.org/10.1016/j.biomaterials.2015.12.026.
    Published Version
    https://doi.org/10.1016/j.biomaterials.2015.12.026
    Keyword
    Gelatin microparticles; N-isopropylacrylamide; Critical size cranial defect; Mesenchymal stem cells; Mineralization
    Type
    Journal article
    Publisher
    Elsevier
    Citable link to this page
    https://hdl.handle.net/1911/94155
    Rights
    This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
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    • Chemical and Biomolecular Engineering Publications [253]
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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map