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dc.contributor.authorButt, Craig M.
Miranda, Marie Lynn
Stapleton, Heather M.
dc.date.accessioned 2017-05-03T18:24:05Z
dc.date.available 2017-05-03T18:24:05Z
dc.date.issued 2016
dc.identifier.citation Butt, Craig M., Miranda, Marie Lynn and Stapleton, Heather M.. "Development of an analytical method to quantify PBDEs, OH-BDEs, HBCDs, 2,4,6-TBP, EH-TBB, and BEH-TEBP in human serum." Analytical and Bioanalytical Chemistry, 408, no. 10 (2016) Springer: 2449-2459. https://doi.org/10.1007/s00216-016-9340-3.
dc.identifier.urihttps://hdl.handle.net/1911/94133
dc.description.abstract Polybrominated diphenyl ethers (PBDEs) flame retardants (FRs) were phased-out in the mid-2000s (penta- and octaBDE) and 2013 (decaBDE); however, their hydroxylated metabolites (OH-BDEs) are still commonly detected in human serum. Today, novel FRs such as Firemaster® 550, a mixture that contains two brominated compounds, EH-TBB and BEH-TEBP are used as replacements for PBDEs in some applications, and there is a need to develop a comprehensive analytical method to assess exposure to both legacy PBDEs and novel FRs. This study developed a solid-phase extraction (SPE)-based method to analyze PBDEs, OH-BDEs, 2,4,6-tribromophenol (TBP), hexabromocylcododecane isomers (HBCDs), EH-TBB, and BEH-TEBP in human serum. Briefly, serum proteins were first denatured with formic acid, and then the target analytes were isolated using a SPE column. Finally, the extract was cleaned and fractioned using a silica SPE column. Method performance was assessed by spiking fetal bovine serum with 1–2 ng of the target analytes, and method accuracy was quantified by comparison to a serum Standard Reference Material (SRM). The developed method showed good recovery and accuracy for all target analytes with the exception of the very low and very high molecular weight PBDE congeners. Using this method, 43 serum samples collected from the Healthy Pregnancy, Healthy Baby Study (HPHB) cohort in Durham, NC, USA were analyzed for FRs. A novel finding was the ubiquitous detection of 2,4,6-TBP, at levels greater than the individual PBDE congeners. Furthermore, 2,4,6-TBP was positively correlated with PBDEs, suggesting that they may have a similar source of exposure, or that 2,4,6-TBP may result from metabolism of PBDEs in vivo.
dc.language.iso eng
dc.publisher Springer
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.
dc.title Development of an analytical method to quantify PBDEs, OH-BDEs, HBCDs, 2,4,6-TBP, EH-TBB, and BEH-TEBP in human serum
dc.type Journal article
dc.citation.journalTitle Analytical and Bioanalytical Chemistry
dc.subject.keywordflame retardants
serum analysis
method development
mass spectrometry
dc.citation.volumeNumber 408
dc.citation.issueNumber 10
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1007/s00216-016-9340-3
dc.identifier.pmcid PMC4996073
dc.identifier.pmid 26864867
dc.type.publication post-print
dc.citation.firstpage 2449
dc.citation.lastpage 2459


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