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dc.contributor.authorJames, Amanda Marie
Baker, Meredith B.
Bao, Gang
Searles, Charles D.
dc.date.accessioned 2017-04-04T17:30:17Z
dc.date.available 2017-04-04T17:30:17Z
dc.date.issued 2017
dc.identifier.citation James, Amanda Marie, Baker, Meredith B., Bao, Gang, et al.. "MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA." Theranostics, 7, no. 3 (2017) 634-646. https://doi.org/10.7150/thno.16840.
dc.identifier.urihttp://hdl.handle.net/1911/94049
dc.description.abstract MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate gene expression and are recognized for their roles both as modulators of disease progression and as biomarkers of disease activity, including neurological diseases, cancer, and cardiovascular disease (CVD). Commonly, miRNA abundance is assessed using quantitative real-time PCR (qRT-PCR), however, qRT-PCR for miRNA can be labor intensive, time consuming, and may lack specificity for detection of mature versus precursor forms of miRNA. Here, we describe a novel double molecular beacon approach to miRNA assessment that can distinguish and quantify mature versus precursor forms of miRNA in a single assay, an essential feature for use of miRNAs as biomarkers for disease. Using this approach, we found that molecular beacons with DNA or combined locked nucleic acid (LNA)-DNA backbones can detect mature and precursor miRNAs (pre-miRNAs) of low (< 1 nM) abundance in vitro. The double molecular beacon assay was accurate in assessing miRNA abundance in a sample containing a mixed population of mature and precursor miRNAs. In contrast, qRT-PCR and the single molecular beacon assay overestimated miRNA abundance. Additionally, the double molecular beacon assay was less labor intensive than traditional qRT-PCR and had 10-25% increased specificity. Our data suggest that the double molecular beacon-based approach is more precise and specific than previous methods, and has the promise of being the standard for assessing miRNA levels in biological samples.
dc.language.iso eng
dc.rights This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.title MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA
dc.type Journal article
dc.citation.journalTitle Theranostics
dc.subject.keywordmolecular beacon
microRNA
microRNA detection
cardiovascular disease
PCR
dc.citation.volumeNumber 7
dc.citation.issueNumber 3
dc.contributor.publisher Ivyspring International Publisher
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.7150/thno.16840
dc.identifier.pmcid PMC5327639
dc.identifier.pmid 28255356
dc.type.publication publisher version
dc.citation.firstpage 634
dc.citation.lastpage 646


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This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license