Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization, and NGS approaches
Author
Khodakov, Dmitriy; Wang, Chunyan; Zhang, David Yu
Date
2016Abstract
Nucleic acid sequence variations have been implicated in many diseases, and reliable detection and quantitation of DNA/RNA biomarkers can inform effective therapeutic action, enabling precision medicine. Nucleic acid analysis technologies being translated into the clinic can broadly be classified into hybridization, PCR, and sequencing, as well as their combinations. Here we review the molecular mechanisms of popular commercial assays, and their progress in translation into in vitro diagnostics.
Citation
Published Version
Keyword
molecular diagnostics; nucleic acid tests; PCR; hybridization; next-generation sequencing
Type
Journal article
Publisher
Citable link to this page
https://hdl.handle.net/1911/93798Rights
This is an open access article under the CC BY-NC-ND licenseLink to License
https://creativecommons.org/licenses/by-nc-nd/4.0/Metadata
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