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dc.contributor.authorLiu, Zheng
Guu, Tom S.Y.
Cao, Jianhao
Li, Yinyin
Cheng, Lingpeng
Tao, Yizhi Jane
Zhang, Jingqiang
dc.date.accessioned 2016-12-16T17:50:26Z
dc.date.available 2016-12-16T17:50:26Z
dc.date.issued 2016
dc.identifier.citation Liu, Zheng, Guu, Tom S.Y., Cao, Jianhao, et al.. "Structure determination of a human virus by the combination of cryo-EM and X-ray crystallography." Biophysics Reports, 2, no. 2 (2016) Springer: 55-68. http://dx.doi.org/10.1007/s41048-016-0027-2.
dc.identifier.urihttps://hdl.handle.net/1911/93724
dc.description.abstract Virus 3D atomic structures provide insight into our understanding of viral life cycles and the development of antiviral drugs. X-ray crystallography and cryo-EM have been used to determine the atomic structure of viruses. However, limited availability of biological samples, biosafety issues due to virus infection, and sometimes inherent characteristics of viruses, pose difficulties on combining both methods in determining viral structures. These have made solving the high resolution structure of some medically important viruses very challenging. Here, we describe our recently employed protocols for determining the high-resolution structure of the virus-like particle of hepatitis E virus (HEV), a pathogen of viral hepatitis in human. These protocols include utilizing recombinant baculovirus system to generate sufficient amount of virus particles, single-particle cryo-EM to get an intermediate resolution structure as a phasing model, and X-ray crystallography for final atomic structure determination. Our protocols have solved the hepatitis E virus structure to the resolution of 3.5 Å. The combined methodology is generally applicable to other human infectious viruses.
dc.language.iso eng
dc.publisher Springer
dc.rights This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.title Structure determination of a human virus by the combination of cryo-EM and X-ray crystallography
dc.type Journal article
dc.citation.journalTitle Biophysics Reports
dc.subject.keywordstructure determination
Cryo-EM
X-ray crystallography
human virus
dc.citation.volumeNumber 2
dc.citation.issueNumber 2
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1007/s41048-016-0027-2
dc.type.publication publisher version
dc.citation.firstpage 55
dc.citation.lastpage 68


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