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    Structure determination of a human virus by the combination of cryo-EM and X-ray crystallography

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    Author
    Liu, Zheng; Guu, Tom S.Y.; Cao, Jianhao; Li, Yinyin; Cheng, Lingpeng; More... Tao, Yizhi Jane; Zhang, Jingqiang Less...
    Date
    2016
    Abstract
    Virus 3D atomic structures provide insight into our understanding of viral life cycles and the development of antiviral drugs. X-ray crystallography and cryo-EM have been used to determine the atomic structure of viruses. However, limited availability of biological samples, biosafety issues due to virus infection, and sometimes inherent characteristics of viruses, pose difficulties on combining both methods in determining viral structures. These have made solving the high resolution structure of some medically important viruses very challenging. Here, we describe our recently employed protocols for determining the high-resolution structure of the virus-like particle of hepatitis E virus (HEV), a pathogen of viral hepatitis in human. These protocols include utilizing recombinant baculovirus system to generate sufficient amount of virus particles, single-particle cryo-EM to get an intermediate resolution structure as a phasing model, and X-ray crystallography for final atomic structure determination. Our protocols have solved the hepatitis E virus structure to the resolution of 3.5 Å. The combined methodology is generally applicable to other human infectious viruses.
    Citation
    Liu, Zheng, Guu, Tom S.Y., Cao, Jianhao, et al.. "Structure determination of a human virus by the combination of cryo-EM and X-ray crystallography." Biophysics Reports, 2, no. 2 (2016) Springer: 55-68. http://dx.doi.org/10.1007/s41048-016-0027-2.
    Published Version
    http://dx.doi.org/10.1007/s41048-016-0027-2
    Keyword
    structure determination; Cryo-EM; X-ray crystallography; human virus
    Type
    Journal article
    Publisher
    Springer
    Citable link to this page
    https://hdl.handle.net/1911/93724
    Rights
    This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
    Link to License
    https://creativecommons.org/licenses/by/4.0/
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    • BioSciences Publications [365]
    • Faculty Publications [4990]

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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map