Show simple item record

dc.contributor.authorShaikh, Sana A.
Dolino, Drew M.
Lee, Garam
Chatterjee, Sudeshna
MacLean, David M.
Flatebo, Charlotte
Landes, Christy F.
Jayaraman, Vasanthi
dc.date.accessioned 2016-12-01T17:29:09Z
dc.date.available 2016-12-01T17:29:09Z
dc.date.issued 2016
dc.identifier.citation Shaikh, Sana A., Dolino, Drew M., Lee, Garam, et al.. "Stargazin Modulation of AMPA Receptors." Cell Reports, 17, no. 2 (2016) Elsevier: 328-335. http://dx.doi.org/10.1016/j.celrep.2016.09.014.
dc.identifier.urihttps://hdl.handle.net/1911/92735
dc.description.abstract Fast excitatory synaptic signaling in the mammalian brain is mediated by AMPA-type ionotropic glutamate receptors. In neurons, AMPA receptors co-assemble with auxiliary proteins, such as stargazin, which can markedly alter receptor trafficking and gating. Here, we used luminescence resonance energy transfer measurements to map distances between the full-length, functional AMPA receptor and stargazin expressed in HEK293 cells and to determine the ensemble structural changes in the receptor due to stargazin. In addition, we used single-molecule fluorescence resonance energy transfer to study the structural and conformational distribution of the receptor and how this distribution is affected by stargazin. Our nanopositioning data place stargazin below the AMPA receptor ligand-binding domain, where it is well poised to act as a scaffold to facilitate the long-range conformational selection observations seen in single-molecule experiments. These data support a model of stargazin acting to stabilize or select conformational states that favor activation.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an open access article under the CC BY-NC-ND license
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.title Stargazin Modulation of AMPA Receptors
dc.type Journal article
dc.citation.journalTitle Cell Reports
dc.contributor.org Applied Physics Graduate Program
dc.subject.keywordAMPA receptors
transmembrane AMPA receptor regulatory proteins
stargazing
AMPA receptor modulation
desensitized state
smFRET
dc.citation.volumeNumber 17
dc.citation.issueNumber 2
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.celrep.2016.09.014
dc.type.publication publisher version
dc.citation.firstpage 328
dc.citation.lastpage 335


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record