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dc.contributor.authorVo, Tiffany N.
Ekenseair, Adam K.
Spicer, Patrick P.
Watson, Brendan M.
Tzouanas, Stephanie N.
Roh, Terrence T.
Mikos, Antonios G.
dc.date.accessioned 2016-08-30T20:50:15Z
dc.date.available 2016-08-30T20:50:15Z
dc.date.issued 2015
dc.identifier.citation Vo, Tiffany N., Ekenseair, Adam K., Spicer, Patrick P., et al.. "In vitroᅠandᅠin vivoᅠevaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineering." Journal of Controlled Release, 205, (2015) Elsevier: 25-34. http://dx.doi.org/10.1016/j.jconrel.2014.11.028.
dc.identifier.urihttps://hdl.handle.net/1911/91365
dc.description.abstract In this study, we investigated the mineralization capacity and biocompatibility of injectable, dual-gelling hydrogels in a rat cranial defect as a function of hydrogel hydrophobicity from either the copolymerization of a hydrolyzable lactone ring or the hydrogel polymer content. The hydrogel system comprised a poly(N-isopropylacrylamide)-based thermogelling macromer (TGM) and a polyamidoamine crosslinker. The thermogelling macromer was copolymerized with (TGM/DBA) or without (TGM) a dimethyl-γ-butyrolactone acrylate (DBA)-containing lactone ring that modulated the lower critical solution temperature and thus, the hydrogel hydrophobicity, over time. Three hydrogel groups were examined: (1) 15wt.% TGM, (2) 15wt.% TGM/DBA, and (3) 20wt.% TGM/DBA. The hydrogels were implanted within an 8mm critical size rat cranial defect for 4 and 12weeks. Implants were harvested at each timepoint and analyzed for bone formation, hydrogel mineralization and tissue response using microcomputed tomography (microCT). Histology and fibrous capsule scoring showed a light inflammatory response at 4weeks that was mitigated by 12weeks for all groups. MicroCT scoring and bone volume quantification demonstrated a similar bone formation at 4weeks that was significantly increased for the more hydrophobic hydrogel formulations - 15wt.% TGM and 20wt.% TGM/DBA - from 4weeks to 12weeks. A complementary in vitro acellular mineralization study revealed that the hydrogels exhibited calcium binding properties in the presence of serum-containing media, which was modulated by the hydrogel hydrophobicity. The tailored mineralization capacity of these injectable, dual-gelling hydrogels with hydrolysis-dependent hydrophobicity presents an exciting property for their use in bone tissue engineering applications.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title In vitroᅠandᅠin vivoᅠevaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineering
dc.type Journal article
dc.citation.journalTitle Journal of Controlled Release
dc.subject.keywordbone
Poly(N-isopropylacrylamide)
thermogelling
tissue engineering
dc.citation.volumeNumber 205
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.jconrel.2014.11.028
dc.identifier.pmcid PMC4395531
dc.identifier.pmid 25483428
dc.type.publication post-print
dc.citation.firstpage 25
dc.citation.lastpage 34


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