Studies on cell synthesis in mixed microbial populations: cell recovery and total biological oxygen demand
Busch, A. W.
Master of Science
A study was conducted in the Environmental Engineering Laboratory of Rice University on the cell recovery phase of the total biological oxygen demand, or TbOD, test, and further experiments were performed to confirm the validity of the total biological oxygen demand theory. Two general methods of cell recovery were investigated, a gravimetric technique and a chemical oxidation technique. TbOD was calculated using the results of each of these techniques as well as by a third method which was based on the chemical oxidation of the substrate itself. It was found that the gravimetric cell recovery technique gave the most accurate and precise results of the methods considered. Consequently, the method of calculating TbOD based on this technique proved to be the best of the methods studied. In general the results obtained were in good agreement with the theory. However, the validity of the assumption of a generalized formula for cell material should be investigated further.