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dc.contributor.advisor Bruner, Robert
dc.creatorElliott, Joel J
dc.date.accessioned 2016-04-21T12:02:07Z
dc.date.available 2016-04-21T12:02:07Z
dc.date.issued 1973
dc.identifier.citation Elliott, Joel J. "DNA replication in Escherichia coli in vivo and in vitro: Effects of bacteriophage T‚ôØ infection." (1973) Master’s Thesis, Rice University. http://hdl.handle.net/1911/89303.
dc.identifier.urihttp://hdl.handle.net/1911/89303
dc.description.abstract DNA synthesis has been studied in an in vitro system derived from cells infected with bacteriophage T4. This in vitro system consists of cells treated with toluene. Such cells, though no longer viable, nevertheless incorporate exogenous deoxyribonucleotide triphosphates into DNA (Moses and Richardson, 1970; Mordoh, et. al., 1970). Toluenization of T4-infected cells results in the reversal of phage-induced shutoff of host DNA synthesis. To see this result, however, the infecting phage must carry a mutation in the D2a region of the T4 genome. If the phage is D2a+ host DNA synthesis in vitro is severely depressed. Thus, the D2a product appears to inhibit, either directly or indirectly, bacterial DNA synthesis in toluenized cells. The D2a product probably controls (or is) a cytosine-specific deoxyribonuclease (Bruner, et. al., 1972). T4 DNA synthesis can occur in toluenized cells. T4 mutants which are defective in host DNA degradation nevertheless shut off host DNA synthesis in vivo.
dc.format.extent 60 pp
dc.language.iso eng
dc.title DNA replication in Escherichia coli in vivo and in vitro: Effects of bacteriophage T‚ôØ infection
dc.identifier.digital RICE0341
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Biology
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Arts
dc.format.digitalOrigin reformatted digital
dc.identifier.callno Thesis Biol. 1973 Elliott


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