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dc.contributor.authorShis, David L.
Hussain, Faiza
Meinhardt, Sarah
Swint-Kruse, Liskin
Bennett, Matthew R.
dc.date.accessioned 2016-02-02T19:17:43Z
dc.date.available 2016-02-02T19:17:43Z
dc.date.issued 2014
dc.identifier.citation Shis, David L., Hussain, Faiza, Meinhardt, Sarah, et al.. "Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras." ACS Synthetic Biology, 3, no. 9 (2014) American Chemical Society: 645-651. http://dx.doi.org/10.1021/sb500262f.
dc.identifier.urihttps://hdl.handle.net/1911/88299
dc.description.abstract In prokaryotes, the construction of synthetic, multi-input promoters is constrained by the number of transcription factors that can simultaneously regulate a single promoter. This fundamental engineering constraint is an obstacle to synthetic biologists because it limits the computational capacity of engineered gene circuits. Here, we demonstrate that complex multi-input transcriptional logic gating can be achieved through the use of ligand-inducible chimeric transcription factors assembled from the LacI/GalR family. These modular chimeras each contain a ligand-binding domain and a DNA-binding domain, both of which are chosen from a library of possibilities. When two or more chimeras have the same DNA-binding domain, they independently and simultaneously regulate any promoter containing the appropriate operator site. In this manner, simple transcriptional AND gating is possible through the combination of two chimeras, and multiple-input AND gating is possible with the simultaneous use of three or even four chimeras. Furthermore, we demonstrate that orthogonal DNA-binding domains and their cognate operators allow the coexpression of multiple, orthogonal AND gates. Altogether, this work provides synthetic biologists with novel, ligand-inducible logic gates and greatly expands the possibilities for engineering complex synthetic gene circuits.
dc.language.iso eng
dc.publisher American Chemical Society
dc.rights This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
dc.rights.urihttp://pubs.acs.org/page/policy/authorchoice_termsofuse.html
dc.title Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras
dc.type Journal article
dc.contributor.funder National Institutes Health
dc.contributor.funder National Science Foundation/ National Institute of General Medical Sciences Mathematical Biology Program
dc.contributor.funder Welch Foundation
dc.citation.journalTitle ACS Synthetic Biology
dc.citation.volumeNumber 3
dc.citation.issueNumber 9
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1021/sb500262f
dc.identifier.pmcid PMC4210161
dc.identifier.pmid 25035932
dc.identifier.grantID R01GM079423 (National Institutes Health)
dc.identifier.grantID R01GM104974 (National Science Foundation/ National Institute of General Medical Sciences Mathematical Biology Program)
dc.identifier.grantID C-1729 (Welch Foundation)
dc.type.publication publisher version
dc.citation.firstpage 645
dc.citation.lastpage 651


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