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dc.contributor.authorLabocha, Marta K.
Jung, Sang-Kyu
Aleman-Meza, Boanerges
Liu, Zheng
Zhong, Weiwei
dc.date.accessioned 2016-02-01T17:23:58Z
dc.date.available 2016-02-01T17:23:58Z
dc.date.issued 2015
dc.identifier.citation Labocha, Marta K., Jung, Sang-Kyu, Aleman-Meza, Boanerges, et al.. "WormGender- Open-Source Software for AutomaticCaenorhabditis elegansᅠSex Ratio Measurement." PLoS ONE, 10, no. 9 (2015) e0139724. http://dx.doi.org/10.1371/journal.pone.0139724.
dc.identifier.urihttp://hdl.handle.net/1911/88294
dc.description.abstractFast and quantitative analysis of animal phenotypes is one of the major challenges of current biology. Here we report the WormGender open-source software, which is designed for accurate quantification of sex ratio in Caenorhabditis elegans. The software functions include, i) automatic recognition and counting of adult hermaphrodites and males, ii) a manual inspection feature that enables manual correction of errors, and iii) flexibility to use new training images to optimize the software for different imaging conditions. We evaluated the performance of our software by comparing manual and automated assessment of sex ratio. Our data showed that the WormGender software provided overall accurate sex ratio measurements. We further demonstrated the usage of WormGender by quantifying the high incidence of male (him) phenotype in 27 mutant strains. Mutants of nine genes (brc-1, C30G12.6, cep-1, coh-3, him-3, him-5, him-8, skr-1, unc-86) showed significant him phenotype. The WormGender is written in Java and can be installed and run on both Windows and Mac platforms. The source code is freely available together with a user manual and sample data at http://www.QuantWorm.org/. The source code and sample data are also available at http://dx.doi.org/10.6084/m9.figshare.1541248.
dc.language.iso eng
dc.rights This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.title WormGender- Open-Source Software for AutomaticCaenorhabditis elegansᅠSex Ratio Measurement
dc.type Journal article
dc.contributor.funder National Institutes of Health
dc.contributor.funder Searle Scholars Program
dc.citation.journalTitle PLoS ONE
dc.citation.volumeNumber 10
dc.citation.issueNumber 9
dc.contributor.publisher Public Library of Science
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0139724
dc.identifier.pmcid PMC4589236
dc.identifier.pmid 26421844
dc.identifier.grantID HG004724 (National Institutes of Health)
dc.identifier.grantID DA018341 (National Institutes of Health)
dc.type.publication publisher version
dc.citation.firstpage e0139724


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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.