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dc.contributor.authorZhao, Yuan
Liu, Wei
Xu, You-Ping
Cao, Jia-Yi
Braam, Janet
Cai, Xin-Zhong
dc.date.accessioned 2016-02-01T17:23:57Z
dc.date.available 2016-02-01T17:23:57Z
dc.date.issued 2013
dc.identifier.citation Zhao, Yuan, Liu, Wei, Xu, You-Ping, et al.. "Genome-wide identification and functional analyses of calmodulin genes inᅠ Solanaceous ᅠspecies." BMC Plant Biology, 13, (2013) BioMed Central: http://dx.doi.org/10.1186/1471-2229-13-70.
dc.identifier.urihttps://hdl.handle.net/1911/88292
dc.description.abstract Background: Calmodulin (CaM) is a major calcium sensor in all eukaryotes. It binds calcium and modulates the activity of a wide range of downstream proteins in response to calcium signals. However, little is known about the CaM gene family in Solanaceous species, including the economically important species, tomato (Solanum lycopersicum), and the gene silencing model plant, Nicotiana benthamiana. Moreover, the potential function of CaM in plant disease resistance remains largely unclear. Results: We performed genome-wide identification of CaM gene families in Solanaceous species. Employing bioinformatics approaches, multiple full-length CaM genes were identified from tomato, N. benthamiana and potato (S. tuberosum) genomes, with tomato having 6 CaM genes, N. benthamiana having 7 CaM genes, and potato having 4 CaM genes. Sequence comparison analyses showed that three tomato genes, SlCaM3/4/5, two potato genes StCaM2/3, and two sets of N. benthamiana genes, NbCaM1/2/3/4 and NbCaM5/6, encode identical CaM proteins, yet the genes contain different intron/exon organization and are located on different chromosomes. Further sequence comparisons and gene structural and phylogenetic analyses reveal that Solanaceous species gained a new group of CaM genes during evolution. These new CaM genes are unusual in that they contain three introns in contrast to only a single intron typical of known CaM genes in plants. The tomato CaM (SlCaM) genes were found to be expressed in all organs. Prediction of cis-acting elements in 5' upstream sequences and expression analyses demonstrated that SlCaM genes have potential to be highly responsive to a variety of biotic and abiotic stimuli. Additionally, silencing of SlCaM2 and SlCaM6 altered expression of a set of signaling and defense-related genes and resulted in significantly lower resistance to Tobacco rattle virus and the oomycete pathogen, Pythium aphanidermatum. Conclusions: The CaM gene families in the Solanaceous species tomato, N. benthamiana and potato were identified through a genome-wide analysis. All three plant species harbor a small set of genes that encode identical CaM proteins, which may manifest a strategy of plants to retain redundancy or enhanced quantitative gene function. In addition, Solanaceous species have evolved one new group of CaM genes during evolution. CaM genes play important roles in plant disease resistance to a variety of pathogens.
dc.language.iso eng
dc.publisher BioMed Central
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttps://creativecommons.org/licenses/by/2.0/
dc.title Genome-wide identification and functional analyses of calmodulin genes inᅠ Solanaceous ᅠspecies
dc.type Journal article
dc.contributor.funder National Basic Research Program of China
dc.contributor.funder Special Fund for Agro-scientific Research in the Public Interest
dc.contributor.funder Program for Changjiang Scholars and Innovative Research Team in University
dc.contributor.funder Program for New Century Excellent Talents in University
dc.contributor.funder Program for New Century 151 Talents of Zhejiang Province
dc.contributor.funder National Science Foundation
dc.citation.journalTitle BMC Plant Biology
dc.subject.keywordCalcium
Calmodulin
Gene Structure
Phylogenetic Analysis
Defense
Resistance
Tomato
Nicotiana Benthamiana
Potato
dc.citation.volumeNumber 13
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2229-13-70
dc.identifier.grantID 2009CB119000 (National Basic Research Program of China)
dc.identifier.grantID 201103016 (Special Fund for Agro-scientific Research in the Public Interest)
dc.identifier.grantID IRT0943 (Program for Changjiang Scholars and Innovative Research Team in University)
dc.identifier.grantID NCET-08-0485 (Program for New Century Excellent Talents in University)
dc.identifier.grantID MCB 0817976 (National Science Foundation)
dc.type.publication publisher version


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