Now showing items 1-20 of 645

    • Multidomain peptide hydrogel adjuvants elicit strong bias towards humoral immunity 

      Pogostin, Brett H.; Yu, Marina H.; Azares, Alon R.; Euliano, Erin M.; Lai, Cheuk Sun Edwin; (2022)
      Adjuvants play a critical role in enhancing vaccine efficacy; however, there is a need to develop new immunomodulatory compounds to address emerging pathogens and to expand the use of immunotherapies. Multidomain peptides (MDPs) are materials composed of canonical amino acids that form injectable supramolecular hydrogels under physiological salt and ...
    • Translating diagnostics and drug delivery technologies to low-resource settings 

      Euliano, Erin M.; Sklavounos, Alexandros A.; Wheeler, Aaron R.; McHugh, Kevin J. (2022)
      Diagnostics and drug delivery technologies engineered for low-resource settings aim to meet their technical design specifications using strategies that are compatible with limited equipment, infrastructure, and operator training. Despite many preclinical successes, very few of these devices have been translated to the clinic. Here, we identify factors ...
    • A unique biomimetic modification endows polyetherketoneketone scaffold with osteoinductivity by activating cAMP/PKA signaling pathway 

      Yuan, Bo; Zhang, Yuxiang; Zhao, Rui; Lin, Hai; Yang, Xiao; (2022)
      Osteoinductivity of a biomaterial scaffold can notably enhance the bone healing performance. In this study, we developed a biomimetic and hierarchically porous polyetherketoneketone (PEKK) scaffold with unique osteoinductivity using a combined surface treatment strategy of a sulfonated process and a nano bone-like apatite deposition. In a beagle ...
    • Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation 

      Chen, Yuda; Jin, Shikai; Zhang, Mengxi; Hu, Yu; Wu, Kuan-Lin; (2022)
      Despite the great promise of genetic code expansion technology to modulate structures and functions of proteins, external addition of ncAAs is required in most cases and it often limits the utility of genetic code expansion technology, especially to noncanonical amino acids (ncAAs) with poor membrane internalization. Here, we report the creation of ...
    • Development of a multimodal mobile colposcope for real-time cervical cancer detection 

      Coole, Jackson B.; Brenes, David; Possati-Resende, Júlio César; Antoniazzi, Márcio; Fonseca, Bruno de Oliveira; (2022)
      Cervical cancer remains a leading cause of cancer death among women in low-and middle-income countries. Globally, cervical cancer prevention programs are hampered by a lack of resources, infrastructure, and personnel. We describe a multimodal mobile colposcope (MMC) designed to diagnose precancerous cervical lesions at the point-of-care without the ...
    • Mean-field model for nematic alignment of self-propelled rods 

      Perepelitsa, Misha; Timofeyev, Ilya; Murphy, Patrick; Igoshin, Oleg A.; Center for Theoretical Biological Physics (2022)
      Self-propelled rods are a facet of the field of active matter relevant to many physical systems ranging in scale from shaken granular media and bacterial alignment to the flocking dynamics of animals. In this paper we develop a model for nematic alignment of self-propelled rods interacting through binary collisions. We avoid phenomenological descriptions ...
    • Direct capture and sequencing reveal ultra-short single-stranded DNA in biofluids 

      Cheng, Lauren Y.; Dai, Peng; Wu, Lucia R.; Patel, Abhijit A.; Zhang, David Yu (2022)
      Cell-free DNA (cfDNA) has become the predominant analyte of liquid biopsy; however, recent studies suggest the presence of subnucleosomal-sized DNA fragments in circulation that are likely single-stranded. Here, we report a method called direct capture and sequencing (DCS) tailored to recover such fragments from biofluids by directly capturing them ...
    • Artificial Soils Reveal Individual Factor Controls on Microbial Processes 

      Del Valle, Ilenne; Gao, Xiaodong; Ghezzehei, Teamrat A.; Silberg, Jonathan J.; Masiello, Caroline A. (2022)
      Soil matrix properties influence microbial behaviors that underlie nutrient cycling, greenhouse gas production, and soil formation. However, the dynamic and heterogeneous nature of soils makes it challenging to untangle the effects of different matrix properties on microbial behaviors. To address this challenge, we developed a tunable artificial soil ...
    • Real-time detection of response regulator phosphorylation dynamics in live bacteria 

      Butcher, Ryan J.; Tabor, Jeffrey J. (2022)
      Bacteria utilize two-component system (TCS) signal transduction pathways to sense and adapt to changing environments. In a typical TCS, a stimulus induces a sensor histidine kinase (SHK) to phosphorylate a response regulator (RR), which then dimerizes and activates a transcriptional response. Here, we demonstrate that oligomerization-dependent ...
    • Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer–promoter interactions 

      Wang, Kaiyuan; Escobar, Mario; Li, Jing; Mahata, Barun; Goell, Jacob; (2022)
      Nuclease-inactivated CRISPR/Cas-based (dCas-based) systems have emerged as powerful technologies to synthetically reshape the human epigenome and gene expression. Despite the increasing adoption of these platforms, their relative potencies and mechanistic differences are incompletely characterized, particularly at human enhancer–promoter pairs. Here, ...
    • Modes of Selection in Tumors as Reflected by Two Mathematical Models and Site Frequency Spectra 

      Kurpas, Monika K.; Kimmel, Marek (2022)
      The tug-of-war model was developed in a series of papers of McFarland and co-authors to account for existence of mutually counteracting rare advantageous driver mutations and more frequent slightly deleterious passenger mutations in cancer. In its original version, it was a state-dependent branching process. Because of its formulation, the tug-of-war ...
    • Evaluation of a Point-of-Care Test for Bilirubin in Malawi 

      Shapiro, Alyssa; Anderson, Jessica; Mtenthaonga, Prince; Kumwenda, Watson; Bond, Meaghan; (2022)
      OBJECTIVES: BiliSpec is a low-cost spectrophotometric reader and disposable paper-based strip to quantify total serum bilirubin from several blood drops. This study was a prospective evaluation of BiliSpec in 2 neonatal wards in Malawi compared with a reference standard bilirubinometer over a large range of bilirubin and hematocrit levels. METHODS: ...
    • Assessing Gq-GPCR–induced human astrocyte reactivity using bioengineered neural organoids 

      Cvetkovic, Caroline; Patel, Rajan; Shetty, Arya; Hogan, Matthew K.; Anderson, Morgan; (2022)
      Astrocyte reactivity can directly modulate nervous system function and immune responses during disease and injury. However, the consequence of human astrocyte reactivity in response to specific contexts and within neural networks is obscure. Here, we devised a straightforward bioengineered neural organoid culture approach entailing transcription ...
    • Multiplexed high-throughput localized electroporation workflow with deep learning–based analysis for cell engineering 

      Patino, Cesar A.; Pathak, Nibir; Mukherjee, Prithvijit; Park, So Hyun; Bao, Gang; (2022)
      Manipulation of cells for applications such as biomanufacturing and cell-based therapeutics involves introducing biomolecular cargoes into cells. However, successful delivery is a function of multiple experimental factors requiring several rounds of optimization. Here, we present a high-throughput multiwell-format localized electroporation device ...
    • Evaluating the physicochemical effects of conjugating peptides into thermogelling hydrogels for regenerative biomaterials applications 

      Pearce, Hannah A; Jiang, Emily Y; Swain, Joseph W R; Navara, Adam M; Guo, Jason L; (2021)
      Thermogelling hydrogels, such as poly(N-isopropylacrylamide) [P(NiPAAm)], provide tunable constructs leveraged in many regenerative biomaterial applications. Recently, our lab developed the crosslinker poly(glycolic acid)-poly(ethylene glycol)-poly(glycolic acid)-di(but-2-yne-1,4-dithiol), which crosslinks P(NiPAAm-co-glycidyl methacrylate) via ...
    • Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces 

      Ameruoso, Andrea; Villegas Kcam, Maria Claudia; Cohen, Katherine Piper; Chappell, James (2022)
      The rise of antibiotic-resistant bacteria represents a major threat to global health, creating an urgent need to discover new antibiotics. Natural products derived from the genus Streptomyces represent a rich and diverse repertoire of chemical molecules from which new antibiotics are likely to be found. However, a major challenge is that the biosynthetic ...
    • Exploring the Photophysical Properties of UiO-67 MOF Doped with Rhenium Carbonyl Complexes 

      Ling, Kexin; Ogle, Meredith M.; Flores, Erick; Godoy, Fernando; Martí, Angel A. (2022)
      Directly coordinating transition metal catalysts to the linkers of stable metal organic frameworks (MOFs) is a sleek solution to increasing the longevity of the catalyst. Photoluminescence metal complexes incorporated in MOFs have risen in interest lately. Particularly, Re(bpydc)(CO)3Cl (bpydc = 2,2’-bipyridine-5,5’-dicarboxylic acid) doped zirconium-based ...
    • Tissue Engineering and Regulatory Science 

      Zhao, Peng; Liu, Wenbo; Tian, Jiaxin; Shi, Xinli; Gu, Xiaosong; (2022)
    • A 3D printable perfused hydrogel vascular model to assay ultrasound-induced permeability 

      Royse, Madison K.; Means, A. Kristen; Calderon, Gisele A.; Kinstlinger, Ian S.; He, Yufang; (2022)
      The development of an in vitro model to study vascular permeability is vital for clinical applications such as the targeted delivery of therapeutics. This work demonstrates the use of a perfusion-based 3D printable hydrogel vascular model as an assessment for endothelial permeability and its barrier function. Aside from providing a platform that more ...
    • Additive manufacturing for the development of optical/photonic systems and components 

      Berglund, Gregory; Wisniowiecki, Anna; Gawedzinski, John; Applegate, Brian; Tkaczyk, Tomasz S. (2022)
      The ambition of this review is to provide an up-to-date synopsis of the state of 3D printing technology for optical and photonic components, to gauge technological advances, and to discuss future opportunities. While a range of approaches have been developed and some have been commercialized, no single approach can yet simultaneously achieve small ...