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Investigations in the lactose repressor core domain: Characterization of the tryptophans and the inducer binding site
The lactose repressor protein, the prototypic genetic control element, contains two tryptophan residues at positions 201 and 220. We have utilized site-specific mutagenesis techniques to generate mutant lac repressors with ...
Characterization and modeling of the pH-regulated expression vector, pSM-10
A new type of plasmid vector has been developed for Escherichia coli. Foreign gene expression can be altered by external pH changes. pSM-10 is the first generation of these plasmid constructs that utilizes the cadA operon ...
MAPK pathways of the yeast Saccharomyces cerevisiae: Cell integrity and filamentation/invasion pathway interaction with the hog pathway
Mitogen Activated Protein Kinase (MAPK) cascades are frequently used signal transduction mechanisms in eukaryotes. Of the five MAPK cascade containing signaling pathways in the budding yeast Saccharomyces cerevisiae , the ...
Cloning and characterization of CMF, a density sensing factor in Dictyostelium discoideum
Conditioned medium factor (CMF), is an 80 kD glycoprotein which is the ligand in a cell density sensing system used by developing Dictyostelium discoideum cells. CMF is slowly secreted by cells when they starve, and the ...