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Synthetic studies towards the total synthesis of discorhabdin C
(1994)
Two synthetic approaches towards the total synthesis of discorhabdin C (3) are described. The general strategy for construction of azacarbocyclic spiro dienone system is to employ an intramolecular para-phenolic alkylation ...
Additional insights into the structure and function of adenosine deaminase
(1998)
Adenosine deaminase is a key enzyme in purine catabolism. The elucidation of the crystal structure and recent analysis of site-directed mutants have provided details on the structure and function of the enzyme. The studies ...
The molecular structure of green fluorescent protein
(1997)
The crystal structure of recombinant wild-type green fluorescent protein (GFP) has been solved to a resolution of 1.9 A by multiwavelength anomalous dispersion phasing methods using selenomethionyl GFP crystals. The protein ...
Mechanistic investigations of novel, irreversible inhibitors of S-adenosylhomocysteine hydrolase
(1992)
Isolation of bovine S-adenosylhomocysteine hydrolase by affinity chromatography unexpectedly yielded two forms of the enzyme. Type A contained 4 moles of NAD$\sp+$/mole of enzyme tetramer, while Type B had only half that ...
Structural studies of membrane lytic peptides: A comparison of magainin and melittin
(1996)
Magainin and melittin are 2 members of a class of small amphipathic helical peptides which act as potent antibiotics or toxins. It has been shown that the peptides in this class interact directly with the lipid bilayer ...
Metabolic engineering and flux analysis of Escherichia coli
(1999)
The advances in molecular biology, genome sequencing and mathematical tools have led to growing interest in metabolic engineering, the goal of which is to redirect metabolic fluxes for industrial and medical purposes. ...
Structural determinants of functional behavior in distal pocket mutants of myoglobin
(1995)
The physiological role of myoglobin depends on the modulation of heme activity by the protein. The hypothesis that functional properties are governed by conserved residues in the distal pocket has been tested by site-directed ...
Syntheses, bioactivities and conformations of peptidomimetics containing 2,3-methanoamino acids
(1994)
Asymmetric syntheses of E- and Z-2,3-methanomethionine (ie E- and Z-cyclo-Met), protected Z-2,3-methanoarginine, and Z-2,3-methanoaspartic acid derivatives have been developed. These functionalized 2,3-methanoamino acids ...
Characterization and modeling of the pH-regulated expression vector, pSM-10
(1992)
A new type of plasmid vector has been developed for Escherichia coli. Foreign gene expression can be altered by external pH changes. pSM-10 is the first generation of these plasmid constructs that utilizes the cadA operon ...
Identification and modeling of protein conformational substates
(1999)
The range of conformations of macromolecules and the dynamic interconversion between conformations is an important part of the relationship between structure and function. The existence of side-chain conformational substates ...