A number of physiological factors and their relation to the solventogenesis mechanism were investigated in both batch and continuous cultures of Clostridium acetobutylicum. The initiation of butanol formation in batch ...

Three key enzymes of the Clostridium acetobutylicum ATCC 824 metabolism, namely thiolase, phosphotransbutyrylase (PTB), and CoA transferase, were purified to homogeneity. Each of these enzymes catalyzes the first reaction ...

The advances in molecular biology, genome sequencing and mathematical tools have led to growing interest in metabolic engineering, the goal of which is to redirect metabolic fluxes for industrial and medical purposes. ...

A new type of plasmid vector has been developed for Escherichia coli. Foreign gene expression can be altered by external pH changes. pSM-10 is the first generation of these plasmid constructs that utilizes the cadA operon ...

One of the major concerns in the utilization of plasmid-containing organisms in fermentation processes is the stability of the plasmid. In general, it is observed that two parameters, the probability of plasmid loss due ...

This study focuses on the application of metabolic engineering techniques to diminish acetate excretion, as a means to enhance recombinant protein productivities. The recombinant model system is based on the formation of ...