Now showing items 1-6 of 6
Experimental evolution of TetX2: Correlating changes in fitness to their structural and functional origins
The study of protein evolution and adaptation resides at the junction between the disciplines of biological chemistry and evolutionary biology. We chose the B. thetaiotaomicron tetracycline resistant enzyme TetX2, as our ...
A library approach to single site and combinatorial residue contributions to dimerization of BNIP3-like transmembrane domains
A poly-leucine transmembrane domain library was randomized at positions corresponding to contact surfaces for a right-handed crossing of two helices to determine the significance of small residues, GxxxG motifs, and hydrogen ...
Structural and Functional Studies of the human cohesin subunits Rad21 and SA2
The cohesin complex is responsible for the fidelity of chromosomal segregation during mitosis. It consists of four core subunits namely Rad21/Mcd1/Sccl, Smc1, Smc3 and one of the yeast Scc3 orthologs SA1 or SA2. Sister ...
Evolutionary fates within a microbial population highlight an essential role for protein folding during natural selection
The fitness function developed in this thesis directly links the physicochemical properties of an enzyme to evolutionary fates in a quantitative and predictive manner through a comparative study of empirical and simulated ...
Negative Feedback Mechanisms Regulating Neurotransmitter Release at the Drosophila Neuromuscular Junction
Homeostasis is an indispensable phenomenon in the maintenance of living organisms. Genetic defects which disrupt negative feedback processes can impact homeostatic regulation, potentially resulting in disease. To uncover ...
A structure-function characterization of the ER membrane protein atlastin
The biogenesis and maintenance of the entire endomembrane system is dependent upon membrane fusion proteins. Mounting evidence indicates that the integral membrane GTPase Atlastin is a membrane fusion protein involved in ...