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Structural consequences of base modifications and metal iron interactions on the anticodon stem-loop from tRNA(Phe) (Escherichia coli)
The structural effects of naturally modified bases of RNA are mostly uncharacterized. The structural effects of two base modifications and of cations in the anticodon stem loop of tRNAPhe (Escherichia coli) were studied ...
The effects of amino acid substitution on apomyoglobin stability, folding intermediates, and holoprotein expression
The stabilities and expression levels of over 150 randomly-generated triple mutants and 100 different rationally constructed single, double, and triple mutants of sperm whale myoglobin have been examined. The effects of ...
Structural and thermodynamic analysis of human PCNA bound to peptides derived from DNA polymerase-delta p66-subunit and flap endonuclease-1 (FEN1) proteins
DNA replication and repair requires the coordination of multiple proteins at the replication fork. Among the proteins found at the replisome, hPCNA (h&barbelow;uman P&barbelow;roliferating C&barbelow;ell N&barbelow;uclear ...
Protein folding, stability, and assembly: A thorough assessment of complex behavior
Proteins play an integral role in virtually every biological process. The function of a protein is determined by its three-dimensional structure. However, the connection between a linear sequence of amino acids and its ...
The molecular bases of tryptophan replacement effects in RecA: A biochemical and biophysical characterization of single- and null- tryptophan mutant Escherichia coli RecA proteins
Recombinational DNA repair is an essential component of DNA metabolism and the proteins required for recombinational repair pathways are highly conserved. The Escherichia coli RecA protein is studied as a prototypic strand ...
Overcoming the challenges of twinning and pseudo symmetry in solving the structure of flavodoxin from Desulfovibrio desulfuricans 29577 by x-ray crystallography
In solving the structure of flavodoxin from Desulfovibrio desulfuricans 29577, crystallographic challenges of twinning and pseudo translational symmetry were encountered. The effects of both challenges were investigated ...