Now showing items 1-10 of 217
Synthetic studies towards the total synthesis of discorhabdin C
Two synthetic approaches towards the total synthesis of discorhabdin C (3) are described. The general strategy for construction of azacarbocyclic spiro dienone system is to employ an intramolecular para-phenolic alkylation ...
THE LUNAR EXOSPHERE
Regulation of neuronal SNARE complexes by complexin and calcium(+2)-synaptotagmin
Synaptic transmission relies on an exquisitely orchestrated series of protein-protein interactions that ultimately results in neurotransmitter release into the synaptic cleft. The final step in this reaction is membrane ...
Additional insights into the structure and function of adenosine deaminase
Adenosine deaminase is a key enzyme in purine catabolism. The elucidation of the crystal structure and recent analysis of site-directed mutants have provided details on the structure and function of the enzyme. The studies ...
The effects of amino acid substitution on apomyoglobin stability, folding intermediates, and holoprotein expression
The stabilities and expression levels of over 150 randomly-generated triple mutants and 100 different rationally constructed single, double, and triple mutants of sperm whale myoglobin have been examined. The effects of ...
Key enzymes in parasite sterol metabolism
The lanosterol synthases from Trypanosoma cruzi, Trypanosoma brucei and Pneumocystis carinii, the lanosterol 14-demethylases from Trypanosoma brucei and Trypanosoma cruzi, and the cytochrome P450 reductase from Trypanosoma ...
Roles of specific and non-specific interactions in folding of beta-sheet and alpha-helical protein model systems
To get a comprehensive understanding of protein folding, the structural complexity of many proteins as well as the properties of the cellular milieu must be considered. For oligomeric proteins, not only is there polypeptide ...
Specificity of membrane helix-helix interactions by mutagenesis and structural analysis
The activity of apoptosis protein BNIP3 has been associated with its ability to form homodimeric and heteromeric associations through its carboxy-terminal transmembrane domain (TMD), but little is known about the chemical ...
The molecular structure of green fluorescent protein
The crystal structure of recombinant wild-type green fluorescent protein (GFP) has been solved to a resolution of 1.9 A by multiwavelength anomalous dispersion phasing methods using selenomethionyl GFP crystals. The protein ...
Predicting protein-protein interactions from primary structure
One of the key challenges in the post-genomic era is to understand protein-protein interactions on a large scale. Given the primary structures of proteins and ligands, along with other information, how well can we ...