Now showing items 1-10 of 217
Nanoshells in vivo imaging using two-photon excitation microscopy
This thesis describes the development and optical characterization of near infrared (NIR) gold nanoshells for the use as luminescent contrast agents for applications in small animal blood vessel imaging. Two types of ...
HEME TRANSPORT AND INCORPORATION INTO GLOBIN
The mechanism of heme uptake and release by cell membranes and proteins was investigated using a model system of carbon monoxide heme, human globin, and phosphatidyl choline single bilayer vesicles. The combination of heme ...
The reactivity of partially reduced metabolities of 2,4,6-trinitrotoluene in natural systems
The reactivity of partially reduced metabolites of 2,4,6-trinitrotoluene (TNT), namely arylhydroxylamines and nitrosoarenes, was evaluated with a simple biological system and with components of soil natural organic matter ...
Regulation of SNARE-mediated membrane fusion by Sec1/Munc18 (SM) proteins
The intricate temporal and spatial regulation of membrane fusion is critical for all living organisms. Fusion of two opposing membranes occurs in a wide range of processes. These include intracellular transportation, ...
ENZYMATIC SYNTHESIS OF THE 2-DEOXYSTREPTAMINE MOIETY OF GENTAMICIN AND RELATED ANTIBIOTICS. CHARACTERIZATION OF A GLUTAMINE: AMINOCYCLITOL AMINOTRANSFERASE
A soluble, stable, L-glutamine:aminocyclitol aminotransferase has been purified 100-fold from mature mycelia of the gentamicin producer Micromonospora purpurea ATCC 15835. An absorbance maximum at 415 nm indicated the ...
Classes of polyadenylation sites revealed by native gel electrophoresis of in vitro assembled complexes and sensitivity to U RNA cleavage
The sequences that comprise a polyadenylation signal are varied. With the exception of the conserved hexanucleotide AAUAAA, other required sequence elements vary from site to site. This variability in sequence content may ...