Now showing items 1-6 of 6
Growth and injury of freely suspended animal cells in an agitated and surface-aerated bioreactor
The mechanism of animal cell damage due to agitation and aeration in a stirred bioreactor and the effect of media components on cell damage were investigated. Hybridoma cells ATCC CRL-8018 were grown in controlled bioreactors ...
Dynamics of plasmid-containing cells in a chemostat under transient conditions
One of the major concerns in the utilization of plasmid-containing organisms in fermentation processes is the stability of the plasmid. In general, it is observed that two parameters, the probability of plasmid loss due ...
Investigations in the lactose repressor core domain: Characterization of the tryptophans and the inducer binding site
The lactose repressor protein, the prototypic genetic control element, contains two tryptophan residues at positions 201 and 220. We have utilized site-specific mutagenesis techniques to generate mutant lac repressors with ...
Purification and studies of two butanol(ethanol) dehydrogenases and the effects of rifampicin and chloramphenicol on other enzymes important in the production of butyrate and butanol in Clostridium acetobutylicum ATCC 824
Two NADH dependent butanol(ethanol) dehydrogenases from Clostridium acetobutylicum have been purified to near homogeneity. Either of these two dehydrogenases could be part of the pathway for the production of butanola and ...
Interactions between lac repressor protein and bromodeoxyuridine-substituted operator DNA: Identification of a specific amino acid-nucleotide contact using UV footprinting and crosslink formation
As the classical model for negative transcriptional control in prokaryotes and the subject of concentrated experimental attention, the lactose operon of Escherichia coli presents a well-defined system for studying genetic ...
Metabolism of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one in 15-ketosterol-adapted cells
5$\alpha$-Cholest-8(14)-en-3$\beta$-ol-15-one is a potent inhibitor of cholesterol biosynthesis in cultured cells. Cells derived from the CHO K-1 cell line have been isolated based on their ability to grow in high ...