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Metabolic engineering and flux analysis of Escherichia coli
The advances in molecular biology, genome sequencing and mathematical tools have led to growing interest in metabolic engineering, the goal of which is to redirect metabolic fluxes for industrial and medical purposes. ...
Growth and injury of freely suspended animal cells in an agitated and surface-aerated bioreactor
The mechanism of animal cell damage due to agitation and aeration in a stirred bioreactor and the effect of media components on cell damage were investigated. Hybridoma cells ATCC CRL-8018 were grown in controlled bioreactors ...
Characterization and application of a periplasmic protein releasing system for extracellular recombinant protein production in Escherichia coli
Escherichia coli is commonly used as a host in the production of high value therapeutic proteins. However, one major drawback associated with E. coli being a host is the intracellular location of product proteins. As a ...
Application of metabolic and biochemical engineering techniques for the enhancement of recombinant protein production in Escherichia coli
This study focuses on the application of metabolic engineering techniques to diminish acetate excretion, as a means to enhance recombinant protein productivities. The recombinant model system is based on the formation of ...
Sensitivity analysis of a mechanistic growth model of Escherichia coli
A detailed mechanistic model, with a large number of metabolites and parameters, describing growth of a single cell of the bacterium Escherichia coli is simulated and the resulting predictions are compared with available ...