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Metabolic engineering and flux analysis of Escherichia coli
The advances in molecular biology, genome sequencing and mathematical tools have led to growing interest in metabolic engineering, the goal of which is to redirect metabolic fluxes for industrial and medical purposes. ...
Characterization and modeling of the pH-regulated expression vector, pSM-10
A new type of plasmid vector has been developed for Escherichia coli. Foreign gene expression can be altered by external pH changes. pSM-10 is the first generation of these plasmid constructs that utilizes the cadA operon ...
Dynamics of plasmid-containing cells in a chemostat under transient conditions
One of the major concerns in the utilization of plasmid-containing organisms in fermentation processes is the stability of the plasmid. In general, it is observed that two parameters, the probability of plasmid loss due ...
Growth and injury of freely suspended animal cells in an agitated and surface-aerated bioreactor
The mechanism of animal cell damage due to agitation and aeration in a stirred bioreactor and the effect of media components on cell damage were investigated. Hybridoma cells ATCC CRL-8018 were grown in controlled bioreactors ...
Mural thrombus and platelet aggregate formation in human blood perfused over collagen or biomaterial surfaces
Epi-fluorescent video microscopy was used to evaluate the effect of ethanol and two nipecotoylpiperazine based antiplatelet agents (BPAT-117 and BPAT-143) on platelet mural thrombus formation. In addition, a series of novel ...
Application of metabolic and biochemical engineering techniques for the enhancement of recombinant protein production in Escherichia coli
This study focuses on the application of metabolic engineering techniques to diminish acetate excretion, as a means to enhance recombinant protein productivities. The recombinant model system is based on the formation of ...