Now showing items 1-5 of 5
Mechanistic investigations of novel, irreversible inhibitors of S-adenosylhomocysteine hydrolase
Isolation of bovine S-adenosylhomocysteine hydrolase by affinity chromatography unexpectedly yielded two forms of the enzyme. Type A contained 4 moles of NAD$\sp+$/mole of enzyme tetramer, while Type B had only half that ...
Characterization and modeling of the pH-regulated expression vector, pSM-10
A new type of plasmid vector has been developed for Escherichia coli. Foreign gene expression can be altered by external pH changes. pSM-10 is the first generation of these plasmid constructs that utilizes the cadA operon ...
Type II dihydrofolate reductases: Probing the natural diversity of enzyme active sites
Type II dihydrofolate reductases (DHFRs) encoded by the R67 and R388 plasmids are sequence and structurally different from known chromosomal DHFRs, and are responsible for conferring trimethoprim resistance to the host ...
Kinetic studies on xanthine oxidase
A comprehensive model for the steady state behavior of xanthine oxidase is proposed. The model includes 36 species which account for all the possible levels of reduction and binding states for the enzyme. A set of seven ...
Crystallographic studies of the maltodextrin-binding protein from Escherichia coli
Crystals were prepared for several studies of the periplasmic maltodextrin-binding protein (MBP) from E. coli. This protein is part of the bacterial transport system for starch metabolites. The complex of MBP with maltotetraose ...