Now showing items 41-50 of 135
High-resolution X-ray structures of myoglobin- and hemoglobin-alkyl isocyanide complexes
The structures of sperm whale myoglobin (Mb) and human hemoglobin (Hb) complexed with methyl, ethyl, n-propyl and n-butyl isocyanide (MNC, ENC, nPNC and nBNC, respectively) were determined by X-ray crystallography. The ...
STUDIES ON RAT KIDNEY GAMMA-GLUTAMYLCYSTEINE SYNTHETASE AND EFFECT OF DIETARY NUCLEOTIDES ON THE IMMUNE FUNCTION OF MICE
An improved purification scheme for rat kidney (gamma)-glutamylcysteinesynthetase ((gamma)GCSase) was developed. Such a preparation yields only onemajor band and one minor band on polyacrylamide gel electrophoresis.Further, ...
METABOLIC STUDIES ON 5ALPHA-CHOLEST-8(14)-EN-3BETA-OL-15-ONE, A POTENT HYPOCHOLESTEROLEMIC AGENT
Part 1. 5(alpha)-Cholest-8(14)-en-3(beta)-ol-15-one, prepared by chemical synthesis, was administered to male rats at a concentration of 0.1% in a low ((TURNEQ)0.005%) cholesterol diet. These rats showed a sustained decrease ...
Growth and injury of freely suspended animal cells in an agitated and surface-aerated bioreactor
The mechanism of animal cell damage due to agitation and aeration in a stirred bioreactor and the effect of media components on cell damage were investigated. Hybridoma cells ATCC CRL-8018 were grown in controlled bioreactors ...
FORMATION AND UTILIZATION OF A SUPPLEMENTAL RESERVOIR OF HIGH-ENERGY PHOSPHATE BY VERTEBRATE BRAIN: CYCLOCREATINE-3-PHOSPHATE
This project has focused on the effects of incorporation of a synthetic analog of creatine-P on the energy metabolism of brain under both in vivo and in vitro conditions. Specifically, it has been shown that the derivative ...
CHEMICAL AND KINETIC STUDIES OF BACTERIAL TRANSPORT AND CHEMORECEPTOR PROTEINS
The L-arabinose-binding protein, an essential component of the high affinity L-arabinose transport system of Escherichia coli, forms a covalent adduct with the chromophoric probe 2-chloromercuri-4-nitrophenol (MNP) at a ...