Now showing items 31-40 of 135
The three-dimensional structure of the D-maltose-binding protein from Escherichia coli
The structure of the sc D-maltose-binding protein, an essential component of the Escherichia coli high affinity osmotic-shock sensitive transport and chemotaxis systems for 1-$\alpha$-4 linked glucose oligosaccharides, has ...
KINETIC STUDIES OF ADENYLOSUCCINATE SYNTHETASE: METAL ACTIVATION, ISOTOPE EXCHANGE AND PULSE-CHASE
Adenylosuccinate synthetase catalyzes the reaction: IMP + aspartate + GTP () AMP-S + GDP + Pi. The enzyme required a divalent cation for activity. When the concentration of GTP was equal to the concentration of the metal ...
ENZYMATIC SYNTHESIS OF THE 2-DEOXYSTREPTAMINE MOIETY OF GENTAMICIN AND RELATED ANTIBIOTICS. CHARACTERIZATION OF A GLUTAMINE: AMINOCYCLITOL AMINOTRANSFERASE
A soluble, stable, L-glutamine:aminocyclitol aminotransferase has been purified 100-fold from mature mycelia of the gentamicin producer Micromonospora purpurea ATCC 15835. An absorbance maximum at 415 nm indicated the ...
Classes of polyadenylation sites revealed by native gel electrophoresis of in vitro assembled complexes and sensitivity to U RNA cleavage
The sequences that comprise a polyadenylation signal are varied. With the exception of the conserved hexanucleotide AAUAAA, other required sequence elements vary from site to site. This variability in sequence content may ...
Interaction of a helical peptide with membrane: Study of alamethicin
Alamethicin is a transmembrane ion channel at low concentration, and a lytic agent of cell membrane at high concentration. It is a small size polypeptide (20 amino residues), and contains a large section of amphiphilic ...
INHIBITORS OF CHOLESTEROL BIOSYNTHESIS: EFFECTS OF 14ALPHA-ETHYL-5ALPHA-CHOLEST-7-ENE-3BETA,15ALPHA-DIOL IN CULTURED MAMMALIAN CELLS. (VOLUMES I AND II) (OXYGENATED STEROLS)
14(alpha)-Ethyl-5(alpha)-cholest-7-ene-3(beta),15(alpha)-diol (0.5 uM) inhibited the synthesis of C(,27) sterols from radioactive acetate in Chinese hamster ovary (CHO-Kl) cells. This inhibition was accompanied by a striking ...