Now showing items 11-20 of 135
Investigations in the lactose repressor core domain: Characterization of the tryptophans and the inducer binding site
The lactose repressor protein, the prototypic genetic control element, contains two tryptophan residues at positions 201 and 220. We have utilized site-specific mutagenesis techniques to generate mutant lac repressors with ...
Kinetic barriers to ligand binding in myoglobin
Picosecond, nanosecond, and bimolecular reactions of ligands with myoglobins containing distal pocket mutations at positions 29, 45, 64, and 68 were examined at 20$\sp\circ$C, in 0.1 M potassium phosphate pH 7.0. The ...
Studies of electron transfer proteins. 1. Spectroscopic studies of cytochrome c peroxidase. 2. Mechanistic and computational studies of cytochrome oxidase
We have measured the magnetic circular dichroism of cytochrome c peroxidase and some of its derivative from 250-350 nm. Comparison of the changes observed on conversion to the catalytic intermediate (cytochrome c peroxidase-I) ...
The molecular structure of green fluorescent protein
The crystal structure of recombinant wild-type green fluorescent protein (GFP) has been solved to a resolution of 1.9 A by multiwavelength anomalous dispersion phasing methods using selenomethionyl GFP crystals. The protein ...
A flexible lattice model to study protein folding
The biological activity of protein molecules is central to life. It has been known for decades that this biological activity is dependent on the protein molecule achieving a particular geometric conformation. Simple lattice ...