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Engineering the Production of Itaconic Acid in Escherichia coli
Itaconic acid (IA) has many versatile applications in science and medicine. Engineering Escherichia coli can provide a reliable route for IA production, where cis -aconitate decarboxylase (CAD) is one essential enzyme in ...
The study of an autocrine signal transduction pathway that represses cell proliferation in Dictyostelium discoideum
Many secreted factors are believed to inhibit cell proliferation in certain tissues such as tumors; however, the identity and action of most of these factors remains unknown. The recent identification of two autocrine ...
Functional and structural studies of the Influenza A virus polymerase subunit PB1 and its complex with the PA subunit
The influenza virus RNA polymerase is a heterotrimeric complex of PA, PB1, and PB2. PB1 functions in RNA elongation, 5' and 3' viral RNA binding, and has an endonuclease activity. For detailed biochemical and structural ...
Monitoring iron-sulfur cluster content of human glutaredoxin 2 using fluorescence proteins
Current methods for directly determining whether an iron-sulfur cluster has been synthesized on a protein in vivo typically require that the protein of interest is purified prior to analysis for bound metallocluster. To ...
AprA: An autocrine, secreted factor that represses cell proliferation in Dictyostelium discoideum
Dictyostelium cells secrete factors during growth and starvation. AprA (autocrine proliferation repressor), a 60 kDa protein secreted as part of a 150 kDa protein complex, functions as a cell proliferation repressor. In ...