Now showing items 1-6 of 6
Analysis of HeLa cell premessenger RNA splicing complexes containing the snRNP U1 by native gel electrophoresis
The typical eukaryotic RNA polymerase II primary transcript is divided into regions that encode information expressed at the protein level (exons) and those which do not (introns). The latter must be removed from the ...
THE RIBOSOMAL RNA INTRONS OF CALLIPHORA ERYTHROCEPHALA AND THE CALMODULIN GENE OF DROSOPHILA MELANOGASTER (INSECTS)
The available cloned examples of the intron-28S coding sequence junctions from the rDNA of Calliphora erythrocephala have been sequenced. These introns interrupt the rDNA at the same position as the type 1 intron family ...
THE ORGANIZATION AND SEQUENCE OF THE 5S RIBOSOMAL GENES OF THE DIPTERAN FLY CALLIPHORA ERYTHROCEPHALA AND THE ISOLATION OF GENES SPECIFICALLY TRANSCRIBED DURING OOGENESIS IN THE DIPTERAN FLY CALLIPHORA ERYTHROCEPHALA
The genomic organization and sequence of the 5S RNA genes of the Dipteran fly Calliphora erythrocephala have been determined. The 5S RNA genes consist of a tandem array of 480 base pair (bp) repeating units which are ...
INDUCTION AND SHUT OFF OF DIHYDROFOLATE REDUCTASE GENE EXPRESSION DURING PRODUCTIVE INFECTION BY ADENOVIRUS TYPE 2
The effects of productive adenovirus infection on host gene expression were studied using a line of methotrexate-resistant HeLa cells containing amplified amounts of the gene coding for dihydrofolate reductase (DHFR). These ...
CONTROL OF GENE EXPRESSION IN ESCHERICHIA COLI THROUGH SUPERCOILING AND OPERATOR REGIONS
This research has investigated the effect that altered supercoiling has on in vivo expression from bacterial promoters. In vivo expression was measured using the pKO system developed by McKenney et al. (1982). Supercoil ...
DNA damage and cell lethality by photodynamically-produced oxygen radicals
Synthetic dyes, including thiazines, acridines, xanthenes, and a phenazine, were used as models for studying the photodynamic effect. In the presence of physiological reductants, the illuminated dyes produced superoxide ...