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Metabolic engineering and flux analysis of Escherichia coli
The advances in molecular biology, genome sequencing and mathematical tools have led to growing interest in metabolic engineering, the goal of which is to redirect metabolic fluxes for industrial and medical purposes. ...
Growth and injury of freely suspended animal cells in an agitated and surface-aerated bioreactor
The mechanism of animal cell damage due to agitation and aeration in a stirred bioreactor and the effect of media components on cell damage were investigated. Hybridoma cells ATCC CRL-8018 were grown in controlled bioreactors ...
Application of metabolic and biochemical engineering techniques for the enhancement of recombinant protein production in Escherichia coli
This study focuses on the application of metabolic engineering techniques to diminish acetate excretion, as a means to enhance recombinant protein productivities. The recombinant model system is based on the formation of ...