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Isolation and characterization of human small nuclear ribonucleoproteins
Human small nuclear ribonucleoproteins (snRNPs) containing U snRNAs have been fractionated into three RNA-specific populations, and snRNPs containing U1 and U2 snRNAs have been isolated~by biochemical methods. U1 and U2 ...
Construction of recombinant molecules containing RNA processing signals and binding of human snRNPs to some nucleic acids
The restriction endonuclease Pst I cleavage site map of Ad2 was generated. Several recombinant molecules were formed, containing Pst I-generated fragments of Ad2 DNA, inserted into the E. coli vector pBR322. These molecules ...
Chemical and spectral studies of the Lac repressor protein and its trypsin resistant core
The core protein produced by mild proteolytic digestion of the lac repressor has been purified on phosphocellulose, The repressor and core proteins were reacted with the sulfhydryl specific reagents, 2-chloromercuri-4-nitrophenol ...
The effect of an oral or intravenous nucleotide-free diet on selected enzyme activities in purine metabolism in rats
Interrelationships between purine metabolism and immunity, cancer, and the diet have been considered. In studying trends of metabolic changes which occur in response to changes in the purine content of the diet, it has ...